![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
Preview |
PDF (Original Article)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
2MB |
![[thumbnail of Supplementary Material]](https://edoc.mdc-berlin.de/style/images/fileicons/other.png) |
Other (Supplementary Material)
1MB |
| Item Type: | Article |
|---|---|
| Title: | Analyses of circRNA expression throughout the light-dark cycle reveal a strong regulation of (Cdr1as), associated with light entrainment in the SCN |
| Creators Name: | Ivanov, A., Mattei, D., Radscheit, K., Compagnion, A.C., Pett, J.P., Herzel, H.P., Paolicelli, R.C., Piwecka, M., Meyer, U. and Beule, D. |
| Abstract: | Circular RNAs (circRNAs) are a large class of relatively stable RNA molecules that are highly expressed in animal brains. Many circRNAs have been associated with CNS disorders accompanied by an aberrant wake-sleep cycle. However, the regulation of circRNAs in brain homeostasis over daily light-dark (LD) cycles has not been characterized. Here, we aim to quantify the daily expression changes of circRNAs in physiological conditions in healthy adult animals. Using newly generated and public RNA-Seq data, we monitored circRNA expression throughout the 12:12 h LD cycle in various mouse brain regions. We identified that (Cdr1as), a conserved circRNA that regulates synaptic transmission, is highly expressed in the suprachiasmatic nucleus (SCN), the master circadian pacemaker. Despite its high stability, (Cdr1as) has a very dynamic expression in the SCN throughout the LD cycle, as well as a significant regulation in the hippocampus following the entry into the dark phase. Computational integration of different public datasets predicted that (Cdr1as) is important for regulating light entrainment in the SCN. We hypothesize that the expression changes of (Cdr1as) in the SCN, particularly during the dark phase, are associated with light-induced phase shifts. Importantly, our work revises the current beliefs about natural circRNA stability and suggests that the time component must be considered when studying circRNA regulation. |
| Keywords: | Circular RNA (circRNA), Cdr1As, Circadian Rhythm, Suprachiasmatic Nucleus, SCN, Non-Coding Rna, Cyrano, Light Entrainment, miR-7, Light-Dark, Wake-Sleep, Animals, Mice |
| Source: | International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| Publisher: | MDPI |
| Volume: | 23 |
| Number: | 20 |
| Page Range: | 12347 |
| Date: | October 2022 |
| Official Publication: | https://doi.org/10.3390/ijms232012347 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
