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| Item Type: | Article |
|---|---|
| Title: | Astrocytes resist hiv-1 fusion but engulf infected macrophage material |
| Creators Name: | Russell, R.A., Chojnacki, J., Jones, D.M., Johnson, E., Do, T., Eggeling, C., Padilla-Parra, S. and Sattentau, Q.J. |
| Abstract: | HIV-1 disseminates to diverse tissues and establishes long-lived viral reservoirs. These reservoirs include the CNS, in which macrophage-lineage cells, and as suggested by many studies, astrocytes, may be infected. Here, we have investigated astrocyte infection by HIV-1. We confirm that astrocytes trap and internalize HIV-1 particles for subsequent release but find no evidence that these particles infect the cell. Astrocyte infection was not observed by cell-free or cell-to-cell routes using diverse approaches, including luciferase and GFP reporter viruses, fixed and live-cell fusion assays, multispectral flow cytometry, and super-resolution imaging. By contrast, we observed intimate interactions between HIV-1-infected macrophages and astrocytes leading to signals that might be mistaken for astrocyte infection using less stringent approaches. These results have implications for HIV-1 infection of the CNS, viral reservoir formation, and antiretroviral therapy. |
| Keywords: | HIV-1, Astrocyte, Fusion, Macrophage, Phagocytosis, Brain |
| Source: | Cell Reports |
| ISSN: | 2211-1247 |
| Publisher: | Cell Press / Elsevier |
| Volume: | 18 |
| Number: | 6 |
| Page Range: | 1473-1483 |
| Date: | 7 February 2017 |
| Official Publication: | https://doi.org/10.1016/j.celrep.2017.01.027 |
| PubMed: | View item in PubMed |
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