Single cell metabolism: current and future trends

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Item Type:	Review
Title:	Single cell metabolism: current and future trends
Creators Name:	Ali, A., Davidson, S., Fraenkel, E., Gilmore, I., Hankemeier, T., Kirwan, J.A., Lane, A.N., Lanekoff, I., Larion, M., McCall, L.I., Murphy, M., Sweedler, J.V. and Zhu, C.
Abstract:	Single cell metabolomics is an emerging and rapidly developing field that complements developments in single cell analysis by genomics and proteomics. Major goals include mapping and quantifying the metabolome in sufficient detail to provide useful information about cellular function in highly heterogeneous systems such as tissue, ultimately with spatial resolution at the individual cell level. The chemical diversity and dynamic range of metabolites poses particular challenges for detection, identification and quantification. In this review we discuss both significant technical issues of measurement and interpretation, and progress toward addressing them, with recent examples from diverse biological systems. We provide a framework for further directions aimed at improving workflow and robustness so that such analyses may become commonly applied, especially in combination with metabolic imaging and single cell transcriptomics and proteomics.
Keywords:	Single Cell Metabolism, Spatial Metabolomics, Metabolic Imaging
Source:	Metabolomics
ISSN:	1573-3882
Publisher:	Springer
Volume:	18
Number:	10
Page Range:	77
Date:	1 October 2022
Additional Information:	Copyright © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022
Official Publication:	https://doi.org/10.1007/s11306-022-01934-3
External Fulltext:	View full text on PubMed Central
PubMed:	View item in PubMed

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