Nanozyme-catalysed CRISPR assay for preamplification-free detection of non-coding RNAs

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Item Type:	Article
Title:	Nanozyme-catalysed CRISPR assay for preamplification-free detection of non-coding RNAs
Creators Name:	Broto, M., Kaminski, M.M., Adrianus, C., Kim, N., Greensmith, R., Dissanayake-Perera, S., Schubert, A.J., Tan, X., Kim, H., Dighe, A.S., Collins, J.J. and Stevens, M.M.
Abstract:	CRISPR-based diagnostics enable specific sensing of DNA and RNA biomarkers associated with human diseases. This is achieved through the binding of guide RNAs to a complementary sequence that activates Cas enzymes to cleave reporter molecules. Currently, most CRISPR-based diagnostics rely on target preamplification to reach sufficient sensitivity for clinical applications. This limits quantification capability and adds complexity to the reaction chemistry. Here we show the combination of a CRISPR-Cas-based reaction with a nanozyme-linked immunosorbent assay, which allows for the quantitative and colorimetric readout of Cas13-mediated RNA detection through catalytic metallic nanoparticles at room temperature (CrisprZyme). We demonstrate that CrisprZyme is easily adaptable to a lateral-flow-based readout and different Cas enzymes and enables the sensing of non-coding RNAs including microRNAs, long non-coding RNAs and circular RNAs. We utilize this platform to identify patients with acute myocardial infarction and to monitor cellular differentiation in vitro and in tissue biopsies from prostate cancer patients. We anticipate that CrisprZyme will serve as a universally applicable signal catalyst for CRISPR-based diagnostics, which will expand the spectrum of targets for preamplification-free, quantitative detection.
Keywords:	Biomarkers, CRISPR-Cas Systems, Circular RNA, DNA, Immunosorbents, MicroRNAs
Source:	Nature Nanotechnology
ISSN:	1748-3387
Publisher:	Nature Publishing Group
Volume:	17
Number:	10
Page Range:	1120-1126
Date:	October 2022
Additional Information:	Copyright © 2022 The Author(s), under exclusive licence to Springer Nature Limited
Official Publication:	https://doi.org/10.1038/s41565-022-01179-0
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