iCLIP: protein-RNA interactions at nucleotide resolution

Tools

Preview

PDF (Original Article) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
962kB

Item Type:	Article
Title:	iCLIP: protein-RNA interactions at nucleotide resolution
Creators Name:	Huppertz, I., Attig, J., D'Ambrogio, A., Easton, L.E., Sibley, C.R., Sugimoto, Y., Tajnik, M., König, J. and Ule, J.
Abstract:	RNA-binding proteins (RBPs) are key players in the post-transcriptional regulation of gene expression. Precise knowledge about their binding sites is therefore critical to unravel their molecular function and to understand their role in development and disease. Individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) identifies protein-RNA crosslink sites on a genome-wide scale. The high resolution and specificity of this method are achieved by an intramolecular cDNA circularization step that enables analysis of cDNAs that truncated at the protein-RNA crosslink sites. Here, we describe the improved iCLIP protocol and discuss critical optimization and control experiments that are required when applying the method to new RBPs.
Keywords:	iCLIP, UV Crosslinking and Immunoprecipitation (CLIP), Protein-RNA Interaction, High-Throughput Sequencing, RNA-Binding Protein, RNA, Post-Transcriptional Regulation
Source:	Methods
ISSN:	1046-2023
Publisher:	Elsevier
Volume:	65
Number:	3
Page Range:	274-87
Date:	February 2014
Official Publication:	https://doi.org/10.1016/j.ymeth.2013.10.011
PubMed:	View item in PubMed

Repository Staff Only: item control page

Download Statistics

Downloads

Downloads per month over past year