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| Item Type: | Article |
|---|---|
| Title: | Best practice standards for circular RNA research |
| Creators: |
Nielsen, A.F.  ORCID: https://orcid.org/0000-0003-2653-4863, Bindereif, A., Bozzoni, I. ORCID: https://orcid.org/0000-0002-3485-8537, Hanan, M., Hansen, T.B., Irimia, M., Kadener, S., Kristensen, L.S. ORCID: https://orcid.org/0000-0002-5980-7939, Legnini, I. ORCID: https://orcid.org/0000-0002-8984-5215, Morlando, M., Jarlstad Olesen, M.T., Pasterkamp, R.J. ORCID: https://orcid.org/0000-0003-1631-6440, Preibisch, S. ORCID: https://orcid.org/0000-0002-0276-494X, Rajewsky, N. ORCID: https://orcid.org/0000-0002-4785-4332, Suenkel, C. ORCID: https://orcid.org/0000-0002-5326-6043 and Kjems, J. ORCID: https://orcid.org/0000-0003-4128-9317
|
| Abstract: | Circular RNAs (circRNAs) are formed in all domains of life and via different mechanisms. There has been an explosion in the number of circRNA papers in recent years; however, as a relatively young field, circRNA biology has an urgent need for common experimental standards for isolating, analyzing, expressing and depleting circRNAs. Here we propose a set of guidelines for circRNA studies based on the authors' experience. This Perspective will specifically address the major class of circRNAs in Eukarya that are generated by a spliceosome-catalyzed back-splicing event. We hope that the implementation of best practice principles for circRNA research will help move the field forward and allow a better functional understanding of this fascinating group of RNAs. |
| Keywords: | RNA Splicing, RNA, Circular RNA |
| Source: | Nature Methods |
| ISSN: | 1548-7091 |
| Publisher: | Nature Publishing Group |
| Volume: | 19 |
| Number: | 10 |
| Page Range: | 1208-1220 |
| Date: | October 2022 |
| Additional Information: | Copyright © 2022. Springer Nature America, Inc. |
| Official Publication: | https://doi.org/10.1038/s41592-022-01487-2 |
| External Fulltext: | View full text on PubMed Central |
| PubMed: | View item in PubMed |
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