Best practice standards for circular RNA research

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Item Type:	Article
Title:	Best practice standards for circular RNA research
Creators Name:	Nielsen, A.F., Bindereif, A., Bozzoni, I., Hanan, M., Hansen, T.B., Irimia, M., Kadener, S., Kristensen, L.S., Legnini, I., Morlando, M., Jarlstad Olesen, M.T., Pasterkamp, R.J., Preibisch, S., Rajewsky, N., Suenkel, C. and Kjems, J.
Abstract:	Circular RNAs (circRNAs) are formed in all domains of life and via different mechanisms. There has been an explosion in the number of circRNA papers in recent years; however, as a relatively young field, circRNA biology has an urgent need for common experimental standards for isolating, analyzing, expressing and depleting circRNAs. Here we propose a set of guidelines for circRNA studies based on the authors' experience. This Perspective will specifically address the major class of circRNAs in Eukarya that are generated by a spliceosome-catalyzed back-splicing event. We hope that the implementation of best practice principles for circRNA research will help move the field forward and allow a better functional understanding of this fascinating group of RNAs.
Keywords:	RNA Splicing, RNA, Circular RNA
Source:	Nature Methods
ISSN:	1548-7091
Publisher:	Nature Publishing Group
Volume:	19
Number:	10
Page Range:	1208-1220
Date:	October 2022
Additional Information:	Copyright © 2022. Springer Nature America, Inc.
Official Publication:	https://doi.org/10.1038/s41592-022-01487-2
External Fulltext:	View full text on PubMed Central
PubMed:	View item in PubMed

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