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The anti-glucocorticoid receptor antibody clone 5E4: raising awareness of unspecific antibody binding

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Item Type:Article
Title:The anti-glucocorticoid receptor antibody clone 5E4: raising awareness of unspecific antibody binding
Creators Name:Ehlers, L., Kirchner, M., Mertins, P., Strehl, C., Buttgereit, F. and Gaber, T.
Abstract:Unspecific antibody binding takes a significant toll on researchers in the form of both the economic burden and the disappointed hopes of promising new therapeutic targets. Despite recent initiatives promoting antibody validation, a uniform approach addressing this issue has not yet been developed. Here, we demonstrate that the anti-glucocorticoid receptor (GR) antibody clone 5E4 predominantly targets two different proteins of approximately the same size, namely AMP deaminase 2 (AMPD2) and transcription intermediary factor 1-beta (TRIM28). This paper is intended to generate awareness of unspecific binding of well-established reagents and advocate the use of more rigorous verification methods to improve antibody quality in the future.
Keywords:Antibody Specificity, Cross-Reactivity, Replication Crisis, Glucocorticoid Receptor
Source:International Journal of Molecular Sciences
ISSN:1422-0067
Publisher:MDPI
Volume:23
Number:9
Page Range:5049
Date:2 May 2022
Official Publication:https://doi.org/10.3390/ijms23095049
PubMed:View item in PubMed

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