![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
Preview |
PDF (Original Article)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
4MB |
![[thumbnail of Supplementary Materials]](https://edoc.mdc-berlin.de/style/images/fileicons/other.png) |
Other (Supplementary Materials)
1MB |
| Item Type: | Article |
|---|---|
| Title: | Sexual dimorphism in extracellular matrix composition and viscoelasticity of the healthy and inflamed mouse brain |
| Creators Name: | Batzdorf, C.S., Morr, A.S., Bertalan, G., Sack, I., Silva, R.V. and Infante-Duarte, C. |
| Abstract: | Magnetic resonance elastography (MRE) has revealed sexual dimorphism in brain stiffness in healthy individuals and multiple sclerosis (MS) patients. In an animal model of MS, named experimental autoimmune encephalomyelitis (EAE), we have previously shown that inflammation-induced brain softening was associated with alterations of the extracellular matrix (ECM). However, it remained unclear whether the brain ECM presents sex-specific properties that can be visualized by MRE. Therefore, here we aimed at quantifying sexual dimorphism in brain viscoelasticity in association with ECM changes in healthy and inflamed brains. Multifrequency MRE was applied to the midbrain of healthy and EAE mice of both sexes to quantitatively map regional stiffness. To define differences in brain ECM composition, the gene expression of the key basement membrane components laminin (Lama4, Lama5), collagen (Col4a1, Col1a1), and fibronectin (Fn1) were investigated by RT-qPCR. We showed that the healthy male cortex expressed less Lama4, Lama5, and Col4a1, but more Fn1 (all p < 0.05) than the healthy female cortex, which was associated with 9% softer properties (p = 0.044) in that region. At peak EAE cortical softening was similar in both sexes compared to healthy tissue, with an 8% difference remaining between males and females (p = 0.006). Cortical Lama4, Lama5 and Col4a1 expression increased 2 to 3-fold in EAE in both sexes while Fn1 decreased only in males (all p < 0.05). No significant sex differences in stiffness were detected in other brain regions. In conclusion, sexual dimorphism in the ECM composition of cortical tissue in the mouse brain is reflected by in vivo stiffness measured with MRE and should be considered in future studies by sex-specific reference values. |
| Keywords: | Multiple Sclerosis, Experimental Autoimmune Encephalomyelitis, Sexual Dimorphism, Brain Viscoelasticity, Magnetic Resonance Elastography, Extracellular Matrix, Cerebral Cortex, Neuroinflammation, Basement Membrane, Animals, Mice |
| Source: | Biology |
| ISSN: | 2079-7737 |
| Publisher: | MDPI |
| Volume: | 11 |
| Number: | 2 |
| Page Range: | 230 |
| Date: | 31 January 2022 |
| Official Publication: | https://doi.org/10.3390/biology11020230 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
