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The Cl(-)-channel TMEM16A is involved in the generation of cochlear Ca(2+) waves and promotes the refinement of auditory brainstem networks in mice

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Item Type:Article
Title:The Cl(-)-channel TMEM16A is involved in the generation of cochlear Ca(2+) waves and promotes the refinement of auditory brainstem networks in mice
Creators Name:Maul, A., Huebner, A.K., Strenzke, N., Moser, T., Rübsamen, R., Jovanovic, S. and Hübner, C.A.
Abstract:Before hearing onset (postnatal day 12 in mice), inner hair cells (IHC) spontaneously fire action potentials thereby driving pre-sensory activity in the ascending auditory pathway. The rate of IHC action potential bursts is modulated by inner supporting cells (ISC) of Kölliker's organ through the activity of the Ca(2+) activated Cl(-) channel TMEM16A (ANO1). Here we show that conditional deletion of Ano1 (Tmem16a) in mice disrupts Ca(2+) waves within Kölliker's organ, reduces the burst firing activity and the frequency-selectivity of auditory brainstem neurons in the medial nucleus of the trapezoid body (MNTB), and also impairs the functional refinement of MNTB projections to the lateral superior olive (LSO). These results reveal the importance of the activity of Kölliker's organ for the refinement of central auditory connectivity. In addition, our study suggests involvement of TMEM16A in the propagation of Ca(2+) waves, which may also apply to other tissues expressing TMEM16A.
Keywords:TMEM16A, ANO1, Cochlea, Kölliker’s Organ, Auditory System, Refinement, Animals, Mice
Source:eLife
ISSN:2050-084X
Publisher:eLife Sciences Publications
Volume:11
Page Range:e72251
Date:24 February 2022
Official Publication:https://doi.org/10.7554/eLife.72251
PubMed:View item in PubMed

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