Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Safe and efficient in vivo hematopoietic stem cell transduction in nonhuman primates using HDAd5/35++ vectors

[thumbnail of Original Article]
Preview
PDF (Original Article) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
2MB

Item Type:Article
Title:Safe and efficient in vivo hematopoietic stem cell transduction in nonhuman primates using HDAd5/35++ vectors
Creators Name:Li, C., Wang, H., Gil, S., Germond, A., Fountain, C., Baldessari, A., Kim, J., Liu, Z., Georgakopoulou, A., Radtke, S., Raskó, T., Pande, A., Chiang, C., Chin, E., Yannaki, E., Izsvak, Z., Papayannopoulou, T., Kiem, H.P. and Lieber, A.
Abstract:We tested a new in vivo hematopoietic stem cell (HSC) transduction/selection approach in rhesus macaques using HSC-tropic, integrating, helper-dependent adenovirus vectors (HDAd5/35++) designed for expression of human γ−globin in red blood cells (RBCs) to treat hemoglobinopathies. We show that HDAd5/35++ vectors preferentially transduce HSCs in vivo after intravenous injection into G-CSF/AMD3100-mobilized animals, and that transduced cells return to the bone marrow and spleen. The approach was well tolerated and activation of proinflammatory cytokines that is usually associated with intravenous adenovirus vector injection, was successfully blunted by pre-treatment with dexamethasone in combination with IL-1 and IL-6 receptor blockers. Using our MGMT(P140K)-based in vivo selection approach, γ-globin(+) RBCs increased in all animals with levels up to 90%. After selection, the percentage of γ-globin(+) RBCs declined most likely due to an immune response against human transgene products. Our biodistribution data indicate that γ-globin(+) RBCs in the periphery were mostly derived from mobilized HSCs that homed to the spleen. Integration site analysis revealed a polyclonal pattern and no genotoxicity related to transgene integrations. This is the first proof-of-concept study in nonhuman primates that in vivo HSC gene therapy could be feasible in humans without the need for high-dose chemotherapy conditioning and HSC transplantation.
Keywords:In Vivo, Hematopoietic Stem Cells, Adenovirus Vector, Nonhuman Primates, Hemoglobinopathies, Gamma Globin, Animals, Rhesus Macaques, Macaca Mulatta
Source:Molecular Therapy - Methods and Clinical Development
ISSN:2329-0501
Publisher:Cell Press
Volume:24
Page Range:127-141
Date:10 March 2022
Additional Information:Erratum in: Mol Ther Methods Clin Dev 24: 127-141.
Official Publication:https://doi.org/10.1016/j.omtm.2021.12.003
PubMed:View item in PubMed

Repository Staff Only: item control page

Downloads

Downloads per month over past year

Open Access
MDC Library