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| Item Type: | Article |
|---|---|
| Title: | Cargo-specific recruitment in clathrin- and dynamin-independent endocytosis |
| Creators: |
Moreno-Layseca, P.  ORCID: https://orcid.org/0000-0002-9456-4178, Jäntti, N.Z. ORCID: https://orcid.org/0000-0003-1547-0390, Godbole, R., Sommer, C., Jacquemet, G. ORCID: https://orcid.org/0000-0002-9286-920X, Al-Akhrass, H. ORCID: https://orcid.org/0000-0002-9630-3000, Conway, J.R.W. ORCID: https://orcid.org/0000-0003-3787-277X, Kronqvist, P., Kallionpää, R.E. ORCID: https://orcid.org/0000-0003-0146-0060, Oliveira-Ferrer, L., Cervero, P. ORCID: https://orcid.org/0000-0002-9417-7733, Linder, S. ORCID: https://orcid.org/0000-0001-8226-2802, Aepfelbacher, M., Zauber, H. ORCID: https://orcid.org/0000-0003-2595-1147, Rae, J., Parton, R.G. ORCID: https://orcid.org/0000-0002-7494-5248, Disanza, A. ORCID: https://orcid.org/0000-0001-5101-2907, Scita, G. ORCID: https://orcid.org/0000-0001-7984-1889, Mayor, S., Selbach, M. ORCID: https://orcid.org/0000-0003-2454-8751, Veltel, S. ORCID: https://orcid.org/0000-0002-4925-3776 and Ivaska, J. ORCID: https://orcid.org/0000-0002-6295-6556
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| Abstract: | Spatially controlled, cargo-specific endocytosis is essential for development, tissue homeostasis and cancer invasion. Unlike cargo-specific clathrin-mediated endocytosis, the clathrin- and dynamin-independent endocytic pathway (CLIC-GEEC, CG pathway) is considered a bulk internalization route for the fluid phase, glycosylated membrane proteins and lipids. While the core molecular players of CG-endocytosis have been recently defined, evidence of cargo-specific adaptors or selective uptake of proteins for the pathway are lacking. Here we identify the actin-binding protein Swiprosin-1 (Swip1, EFHD2) as a cargo-specific adaptor for CG-endocytosis. Swip1 couples active Rab21-associated integrins with key components of the CG-endocytic machinery - Arf1, IRSp53 and actin - and is critical for integrin endocytosis. Through this function, Swip1 supports integrin-dependent cancer-cell migration and invasion, and is a negative prognostic marker in breast cancer. Our results demonstrate a previously unknown cargo selectivity for the CG pathway and a role for specific adaptors in recruitment into this endocytic route. |
| Keywords: | Actins, Biological Transport, Breast Neoplasms, Cell Movement, Clathrin, Dynamins, Endocytosis, Integrin beta1, Intracellular Signaling Peptides and Proteins, rab GTP-Binding Proteins |
| Source: | Nature Cell Biology |
| ISSN: | 1465-7392 |
| Publisher: | Nature Publishing Group |
| Volume: | 23 |
| Number: | 10 |
| Page Range: | 1073-1084 |
| Date: | October 2021 |
| Additional Information: | "Erratum in: Nat Cell Biol https://doi.org/10.1038/s41556-021-00767-x, published online 6 October 2021." - Copyright © 2021, The Author(s), under exclusive licence to Springer Nature Limited, corrected publication 2022. |
| Official Publication: | https://doi.org/10.1038/s41556-021-00767-x |
| External Fulltext: | View full text on external repository or document server |
| PubMed: | View item in PubMed |
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