De novo protein structure determination from near-atomic-resolution cryo-EM maps

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Item Type:	Article
Title:	De novo protein structure determination from near-atomic-resolution cryo-EM maps
Creators Name:	Wang, R.Y.R., Kudryashev, M., Li, X., Egelman, E.H., Basler, M., Cheng, Y., Baker, D. and DiMaio, F.
Abstract:	We present a de novo model-building approach that combines predicted backbone conformations with side-chain fit to density to accurately assign sequence into density maps. This method yielded accurate models for six of nine experimental maps at 3.3- to 4.8-Å resolution and produced a nearly complete model for an unsolved map containing a 660-residue heterodimeric protein. This method should enable rapid and reliable protein structure determination from near-atomic-resolution cryo-electron microscopy (cryo-EM) maps.
Keywords:	Cryoelectron Microscopy, Molecular Models, Monte Carlo Method, Physical Chemistry, Protein Conformation, Proteins, Time Factors
Source:	Nature Methods
ISSN:	1548-7091
Publisher:	Nature Publishing Group
Volume:	12
Number:	4
Page Range:	335-338
Date:	April 2015
Official Publication:	https://doi.org/10.1038/nmeth.3287
PubMed:	View item in PubMed

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