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| Item Type: | Article |
|---|---|
| Title: | The zinc finger antiviral protein ZAP restricts human cytomegalovirus and selectively binds and destabilizes viral UL4/UL5 transcripts |
| Creators Name: | Gonzalez-Perez, A.C., Stempel, M., Wyler, E., Urban, C., Piras, A., Hennig, T., Ganskih, S., Wei, Y., Heim, A., Landthaler, M., Pichlmair, A., Dölken, L., Munschauer, M., Erhard, F. and Brinkmann, M.M. |
| Abstract: | Interferon-stimulated gene products (ISGs) play a crucial role in early infection control. The ISG zinc finger CCCH-type antiviral protein 1 (ZAP/ZC3HAV1) antagonizes several RNA viruses by binding to CG-rich RNA sequences, whereas its effect on DNA viruses is less well understood. Here, we decipher the role of ZAP in the context of human cytomegalovirus (HCMV) infection, a β-herpesvirus that is associated with high morbidity in immunosuppressed individuals and newborns. We show that expression of the two major isoforms of ZAP, ZAP-S and ZAP-L, is induced during HCMV infection and that both negatively affect HCMV replication. Transcriptome and proteome analyses demonstrated that the expression of ZAP results in reduced viral mRNA and protein levels and decelerates the progression of HCMV infection. Metabolic RNA labeling combined with high-throughput sequencing (SLAM-seq) revealed that most of the gene expression changes late in infection result from the general attenuation of HCMV. Furthermore, at early stages of infection, ZAP restricts HCMV by destabilizing a distinct subset of viral mRNAs, particularly those from the previously uncharacterized HCMV gene locus. Through enhanced cross-linking immunoprecipitation and sequencing analysis (eCLIP-seq), we identified the transcripts expressed from this HCMV locus as the direct targets of ZAP. Moreover, our data show that ZAP preferentially recognizes not only CG, but also other cytosine-rich sequences, thereby expanding its target specificity. In summary, this report is the first to reveal direct targets of ZAP during HCMV infection, which strongly indicates that transcripts from the locus may play an important role for HCMV replication. |
| Keywords: | ZAP, ZC3HAV1, ISG, Antiviral, DNA Virus, Herpesvirus, HCMV, Innate Immunity, Human Cytomegalovirus, Interferons, mRNA Degradation, Pattern Recognition Receptors, Zinc Finger Proteins |
| Source: | mBio |
| ISSN: | 2150-7511 |
| Publisher: | American Society for Microbiology |
| Volume: | 12 |
| Number: | 3 |
| Page Range: | e02683-20 |
| Date: | 4 May 2021 |
| Official Publication: | https://doi.org/10.1128/mBio.02683-20 |
| PubMed: | View item in PubMed |
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