Genome-wide analysis of actively translated open reading frames using riboTaper/ORFquant

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Item Type:	Article
Title:	Genome-wide analysis of actively translated open reading frames using riboTaper/ORFquant
Creators Name:	Harnett, D., Meerdink, E., Calviello, L., Sydow, D. and Ohler, U.
Abstract:	Ribosome profiling, or Ribo-seq, provides precise information about the position of actively translating ribosomes. It can be used to identify open reading frames (ORFs) that are translated in a given sample. The RiboTaper pipeline, and the ORFquant R package, leverages the periodic distribution of such ribosomes along the ORF to perform a statistically robust test for translation which is insensitive to aperiodic noise and provides a statistically robust measure of translation. In addition to accounting for complex loci with overlapping ORFs, ORFquant is also able to use Ribo-seq as a tool for distinguishing actively translated transcripts from non-translated ones, within a given gene locus.
Keywords:	Ribo-seq, Translation, Genomics, Open Reading Frame, Periodicity, Sequencing, Ribosome, Animals
Source:	Methods in Molecular Biology
ISSN:	1064-3745
Publisher:	Springer / Humana Press
Volume:	2252
Page Range:	331-346
Date:	2021
Official Publication:	https://doi.org/10.1007/978-1-0716-1150-0_16
PubMed:	View item in PubMed

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