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Identification of leukemic and pre-leukemic stem cells by clonal tracking from single-cell transcriptomics

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Item Type:Article
Title:Identification of leukemic and pre-leukemic stem cells by clonal tracking from single-cell transcriptomics
Creators Name:Velten, L., Story, B.A., Hernández-Malmierca, P., Raffel, S., Leonce, D.R., Milbank, J., Paulsen, M., Demir, A., Szu-Tu, C., Frömel, R., Lutz, C., Nowak, D., Jann, J.C., Pabst, C., Boch, T., Hofmann, W.K., Müller-Tidow, C., Trumpp, A., Haas, S. and Steinmetz, L.M.
Abstract:Cancer stem cells drive disease progression and relapse in many types of cancer. Despite this, a thorough characterization of these cells remains elusive and with it the ability to eradicate cancer at its source. In acute myeloid leukemia (AML), leukemic stem cells (LSCs) underlie mortality but are difficult to isolate due to their low abundance and high similarity to healthy hematopoietic stem cells (HSCs). Here, we demonstrate that LSCs, HSCs, and pre-leukemic stem cells can be identified and molecularly profiled by combining single-cell transcriptomics with lineage tracing using both nuclear and mitochondrial somatic variants. While mutational status discriminates between healthy and cancerous cells, gene expression distinguishes stem cells and progenitor cell populations. Our approach enables the identification of LSC-specific gene expression programs and the characterization of differentiation blocks induced by leukemic mutations. Taken together, we demonstrate the power of single-cell multi-omic approaches in characterizing cancer stem cells.
Keywords:Acute Myeloid Leukaemia, Cancer Stem Cells, Haematopoiesis, Transcriptomics, Tumour Heterogeneity
Source:Nature Communications
ISSN:2041-1723
Publisher:Nature Publishing Group
Volume:12
Number:1
Page Range:1366
Date:1 March 2021
Official Publication:https://doi.org/10.1038/s41467-021-21650-1
PubMed:View item in PubMed

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