Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization

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Item Type:	Article
Title:	Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization
Creators Name:	Nikolay, R., Hilal, T., Schmidt, S., Qin, B., Schwefel, D., Vieira-Vieira, C.H., Mielke, T., Bürger, J., Loerke, J., Amikura, K., Flügel, T., Ueda, T., Selbach, M., Deuerling, E. and Spahn, C.M.T.
Abstract:	Ribosome biogenesis is a fundamental multi-step cellular process that culminates in the formation of ribosomal subunits, whose production and modification are regulated by numerous biogenesis factors. In this study, we analyze physiologic prokaryotic ribosome biogenesis by isolating bona fide pre-50S subunits from an Escherichia coli strain with the biogenesis factor ObgE, affinity tagged at its native gene locus. Our integrative structural approach reveals a network of interacting biogenesis factors consisting of YjgA, RluD, RsfS, and ObgE on the immature pre-50S subunit. In addition, our study provides mechanistic insight into how the GTPase ObgE, in concert with other biogenesis factors, facilitates the maturation of the 50S functional core and reveals both conserved and divergent evolutionary features of ribosome biogenesis between prokaryotes and eukaryotes.
Keywords:	Ribosome, Ribosome Assembly, Ribosome Biogenesis, 50S Subunit, ObgE, RluD, RsfS, YjgA, Cryo-Electron Microscopy, Structure
Source:	Molecular Cell
ISSN:	1097-2765
Publisher:	Cell Press
Volume:	81
Number:	6
Page Range:	1200-1215
Date:	18 March 2021
Official Publication:	https://doi.org/10.1016/j.molcel.2021.02.006
PubMed:	View item in PubMed

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