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| Item Type: | Article |
|---|---|
| Title: | Molecular recording of mammalian embryogenesis |
| Creators Name: | Chan, M.M., Smith, Z.D., Grosswendt, S., Kretzmer, H., Norman, T.M., Adamson, B., Jost, M., Quinn, J.J., Yang, D., Jones, M.G., Khodaverdian, A., Yosef, N., Meissner, A. and Weissman, J.S. |
| Abstract: | Ontogeny describes the emergence of complex multicellular organisms from single totipotent cells. This field is particularly challenging in mammals, owing to the indeterminate relationship between self-renewal and differentiation, variation in progenitor field sizes, and internal gestation in these animals. Here we present a flexible, high-information, multi-channel molecular recorder with a single-cell readout and apply it as an evolving lineage tracer to assemble mouse cell-fate maps from fertilization through gastrulation. By combining lineage information with single-cell RNA sequencing profiles, we recapitulate canonical developmental relationships between different tissue types and reveal the nearly complete transcriptional convergence of endodermal cells of extra-embryonic and embryonic origins. Finally, we apply our cell-fate maps to estimate the number of embryonic progenitor cells and their degree of asymmetric partitioning during specification. Our approach enables massively parallel, high-resolution recording of lineage and other information in mammalian systems, which will facilitate the construction of a quantitative framework for understanding developmental processes. |
| Keywords: | Cell Differentiation, Cell Lineage, Developmental Gene Expression Regulation, Embryonic Development, Embryonic Stem Cells, Endoderm, Fertilization, Gastrulation, Mammalian Embryo, Organ Specificity, Phenotype, RNA Sequence Analysis, Single-Cell Analysis, Animals, Mice |
| Source: | Nature |
| ISSN: | 0028-0836 |
| Publisher: | Nature Publishing Group |
| Volume: | 570 |
| Number: | 7759 |
| Page Range: | 77-82 |
| Date: | 6 June 2019 |
| Official Publication: | https://doi.org/10.1038/s41586-019-1184-5 |
| External Fulltext: | View full text on PubMed Central |
| PubMed: | View item in PubMed |
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