A CRISPR-based assay for the detection of opportunistic infections post-transplantation and for the monitoring of transplant rejection

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Item Type:	Article
Title:	A CRISPR-based assay for the detection of opportunistic infections post-transplantation and for the monitoring of transplant rejection
Creators Name:	Kaminski, M.M., Alcantar, M.A., Lape, I.T., Greensmith, R., Huske, A.C., Valeri, J.A., Marty, F.M., Klämbt, V., Azzi, J., Akalin, E., Riella, L.V. and Collins, J.J.
Abstract:	In organ transplantation, infection and rejection are major causes of graft loss. They are linked by the net state of immunosuppression. To diagnose and treat these conditions earlier, and to improve long-term patient outcomes, refined strategies for the monitoring of patients after graft transplantation are needed. Here, we show that a fast and inexpensive assay based on CRISPR-Cas13 accurately detects BK polyomavirus DNA and cytomegalovirus DNA from patient-derived blood and urine samples, as well as CXCL9 messenger RNA (a marker of graft rejection) at elevated levels in urine samples from patients experiencing acute kidney transplant rejection. The assay, which we adapted for lateral-flow readout, enables-via simple visualization-the post-transplantation monitoring of common opportunistic viral infections and of graft rejection, and should facilitate point-of-care post-transplantation monitoring.
Keywords:	Biomarkers, CRISPR-Cas Systems, Chemokine CXCL9, Clustered Regularly Interspaced Short Palindromic Repeats, Cytomegalovirus, Cytomegalovirus Infections, Graft Rejection, Kidney, Kidney Diseases, Kidney Transplantation, Messenger RNA, Molecular Pathology, Opportunistic Infections, Point-of-Care Testing, Polyomavirus, Polyomavirus Infections, Postoperative Complications, Tumor Virus Infections, Viral DNA
Source:	Nature Biomedical Engineering
ISSN:	2157-846X
Publisher:	Nature Publishing Group
Volume:	4
Number:	6
Page Range:	601-609
Date:	June 2020
Additional Information:	Copyright © The Author(s), under exclusive licence to Springer Nature Limited 2020
Official Publication:	https://doi.org/10.1038/s41551-020-0546-5
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