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| Item Type: | Article |
|---|---|
| Title: | An activatable lanthanide luminescent probe for time-gated detection of nitroreductase in live bacteria |
| Creators Name: | Brennecke, B., Wang, Q., Zhang, Q., Hu, H.Y. and Nazaré, M. |
| Abstract: | The development of a turn-on lanthanide luminescent probe for time-gated detection of nitroreductases (NTRs) in live bacteria is reported. The probe is activated through NTR-induced formation of the sensitizing carbostyril antenna and resulting energy transfer to the lanthanide center. This novel NTR-responsive trigger is virtually non-fluorescent in its inactivated form and features a large signal increase upon activation. We show that the probe is capable of selectively sensing NTR in lysates as well as in live bacteria comprising clinically highly relevant multiresistant pathogens of the ESKAPE family responsible for the majority of hospital infections. The results suggest that our probe could be used to develop diagnostic tools for bacterial infections. |
| Keywords: | Bacterial Imaging, Enzymes, Lanthanides, Luminescence, Nitroreductase |
| Source: | Angewandte Chemie International Edition |
| ISSN: | 1433-7851 |
| Publisher: | Wiley-VCH |
| Volume: | 59 |
| Number: | 22 |
| Page Range: | 8512-8516 |
| Date: | 25 May 2020 |
| Official Publication: | https://doi.org/10.1002/anie.202002391 |
| PubMed: | View item in PubMed |
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