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Label-free detection of global morphology changes in confluent cell layers utilizing quantitative phase imaging with digital holographic microscopy

Item Type:Conference or Workshop Item
Title:Label-free detection of global morphology changes in confluent cell layers utilizing quantitative phase imaging with digital holographic microscopy
Creators Name:Kemper, B., Pohl, L., Kaiser, M., Döpker, E., Schnekenburger, J. and Ketelhut, S.
Abstract:For the example of digital holographic microscopy (DHM), a variant of quantitative phase imaging (QPI), we explored strategies for the analysis of confluent cell layers utilizing histogram based-evaluation of quantitative phase images. The applicability of the proposed numerical evaluation procedures is illustrated by the DHM-based quantification of drug induced cell morphology changes. Our results show that histogram-based evaluation of quantitative phase images enables a highly reliable quantitative observation of global cellular morphology changes in confluent cell layers.
Keywords:Quantitative Phase Imaging, Digital Holographic Microscopy, Label-Free Live Cell Imaging, Confluent Cell Layers
Source:Proceedings of SPIE
Title of Book:Advances in Microscopic Imaging II
ISSN:0277-786X
Publisher:International Society for Optics and Photonics (SPIE)
Volume:11076
Page Range:30
Date:22 July 2019
Official Publication:https://doi.org/10.1117/12.2527189

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