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| Item Type: | Article |
|---|---|
| Title: | Linescan microscopy data to extract diffusion coefficient of a fluorescent species using a commercial confocal microscope |
| Creators Name: | Bathe-Peters, M., Gmach, P., Annibale, P. and Lohse, M.J. |
| Abstract: | We report here on the measurement of the diffusion coefficient of fluorescent species using a commercial microscope possessing a resonant scanner. Sequential linescans with a rate of up to 12 kHz yield a temporal resolution of 83 μs, making the setup amenable to measure diffusion rates over a range covering at least three orders of magnitude, from 100 μm(2)/s down to 0.1 μm(2)/s. We share representative data sets covering (i) the diffusion of a dye molecule, observed in media of different viscosities and (ii) the diffusion of a prototypical membrane receptor. The data can be valuable for researchers interested in the rapid diffusion properties of nuclear, cytosolic or membrane bound proteins fused to fluorescent tags. |
| Keywords: | Confocal Microscopy, Fluorescence Correlation Spectroscopy, Membrane Receptor, Diffusion |
| Source: | Data in Brief |
| ISSN: | 2352-3409 |
| Publisher: | Elsevier |
| Volume: | 29 |
| Page Range: | 105063 |
| Date: | April 2020 |
| Official Publication: | https://doi.org/10.1016/j.dib.2019.105063 |
| PubMed: | View item in PubMed |
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