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Linescan microscopy data to extract diffusion coefficient of a fluorescent species using a commercial confocal microscope

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Item Type:Article
Title:Linescan microscopy data to extract diffusion coefficient of a fluorescent species using a commercial confocal microscope
Creators Name:Bathe-Peters, M., Gmach, P., Annibale, P. and Lohse, M.J.
Abstract:We report here on the measurement of the diffusion coefficient of fluorescent species using a commercial microscope possessing a resonant scanner. Sequential linescans with a rate of up to 12 kHz yield a temporal resolution of 83 μs, making the setup amenable to measure diffusion rates over a range covering at least three orders of magnitude, from 100 μm(2)/s down to 0.1 μm(2)/s. We share representative data sets covering (i) the diffusion of a dye molecule, observed in media of different viscosities and (ii) the diffusion of a prototypical membrane receptor. The data can be valuable for researchers interested in the rapid diffusion properties of nuclear, cytosolic or membrane bound proteins fused to fluorescent tags.
Keywords:Confocal Microscopy, Fluorescence Correlation Spectroscopy, Membrane Receptor, Diffusion
Source:Data in Brief
ISSN:2352-3409
Publisher:Elsevier
Volume:29
Page Range:105063
Date:April 2020
Official Publication:https://doi.org/10.1016/j.dib.2019.105063
PubMed:View item in PubMed

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