Item Type: | Article |
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Title: | Isolation and long-term cultivation of mouse alveolar macrophages |
Creators Name: | Busch, C. J.-L., Favret, J., Geirsdóttir, L., Molawi, K. and Sieweke, M.H. |
Abstract: | Alveolar macrophages (AM) are tissue-resident macrophages that colonize the lung around birth and can self-maintain long-term in an adult organism without contribution of monocytes. AM are located in the pulmonary alveoli and can be harvested by washing the lungs using the method of bronchoalveolar lavage (BAL). Here, we compared different conditions of BAL to obtain high yields of murine AM for in vitro culture and expansion of AM. In addition, we describe specific culture conditions, under which AM proliferate long-term in liquid culture in the presence of granulocyte-macrophage colony-stimulating factor. This method can be used to obtain large numbers of AM for in vivo transplantation or for in vitro experiments with primary mouse macrophages. |
Keywords: | Macrophage, Alveolar, Lungs, Self-Renewal, Bronchoalveolar Lavage, Primary Cell Culture, Animals, Mice |
Source: | Bio-protocol |
ISSN: | 2331-8325 |
Publisher: | Bio-protocol LLC |
Volume: | 9 |
Number: | 14 |
Date: | 20 July 2019 |
Additional Information: | Copyright © 2019 The Authors; exclusive licensee Bio-protocol LLC. |
Official Publication: | https://doi.org/10.21769/BioProtoc.3302 |
External Fulltext: | View full text on PubMed Central |
PubMed: | View item in PubMed |
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