Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

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Item Type:	Article
Title:	Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
Creators Name:	Cossarizza, A., Chang, H.D., Radbruch, A., Acs, A., Adam, D., Adam-Klages, S., Agace, W.W., Aghaeepour, N., Akdis, M., Allez, M., Almeida, L.N., Alvisi, G., Anderson, G., Andrä, I., Annunziato, F., Anselmo, A., Bacher, P., Baldari, C.T., Bari, S., Barnaba, V., Barros-Martins, J., Battistini, L., Bauer, W., Baumgart, S., Baumgarth, N., Baumjohann, D., Baying, B., Bebawy, M., Becher, B., Beisker, W., Benes, V., Beyaert, R., Blanco, A., Boardman, D.A., Bogdan, C., Borger, J.G., Borsellino, G., Boulais, P.E., Bradford, J.A., Brenner, D., Brinkman, R.R., Brooks, A.E.S., Busch, D.H., Büscher, M., Bushnell, T.P., Calzetti, F., Cameron, G., Cammarata, I., Cao, X., Cardell, S.L., Casola, S., Cassatella, M.A., Cavani, A., Celada, A., Chatenoud, L., Chattopadhyay, P.K., Chow, S., Christakou, E., Čičin-Šain, L., Clerici, M., Colombo, F.S., Cook, L., Cooke, A., Cooper, A.M., Corbett, A.J., Cosma, A., Cosmi, L., Coulie, P.G., Cumano, A., Cvetkovic, L., Dang, V.D., Dang-Heine, C., Davey, M.S., Davies, D., De Biasi, S., Del Zotto, G., Dela Cruz, G.V., Delacher, M., Della Bella, S., Dellabona, P., Deniz, G., Dessing, M., Di Santo, J.P., Diefenbach, A., Dieli, F., Dolf, A., Dörner, T., Dress, R.J., Dudziak, D., Dustin, M., Dutertre, C.A., Ebner, F., Eckle, S.B.G., Edinger, M., Eede, P., Ehrhardt, G.R.A., Eich, M., Engel, P., Engelhardt, B., Erdei, A., Esser, C., Everts, B., Evrard, M., Falk, C.S., Fehniger, T.A., Felipo-Benavent, M., Ferry, H., Feuerer, M., Filby, A., Filkor, K., Fillatreau, S., Follo, M., Förster, I., Foster, J., Foulds, G.A., Frehse, B., Frenette, P.S., Frischbutter, S., Fritzsche, W., Galbraith, D.W., Gangaev, A., Garbi, N., Gaudilliere, B., Gazzinelli, R.T., Geginat, J., Gerner, W., Gherardin, N.A., Ghoreschi, K., Gibellini, L., Ginhoux, F., Goda, K., Godfrey, D.I., Goettlinger, C., González-Navajas, J.M., Goodyear, C.S., Gori, A., Grogan, J.L., Grummitt, D., Grützkau, A., Haftmann, C., Hahn, J., Hammad, H., Hämmerling, G., Hansmann, L., Hansson, G., Harpur, C.M., Hartmann, S., Hauser, A., Hauser, A.E., Haviland, D.L., Hedley, D., Hernández, D.C., Herrera, G., Herrmann, M., Hess, C., Höfer, T., Hoffmann, P., Hogquist, K., Holland, T., Höllt, T., Holmdahl, R., Hombrink, P., Houston, J.P., Hoyer, B.F., Huang, B., Huang, F.P., Huber, J.E., Huehn, J., Hundemer, M., Hunter, C.A., Hwang, W.Y.K., Iannone, A., Ingelfinger, F., Ivison, S.M., Jäck, H.M., Jani, P.K., Jávega, B., Jonjic, S., Kaiser, T., Kalina, T., Kamradt, T., Kaufmann, S.H.E., Keller, B., Ketelaars, S.L.C., Khalilnezhad, A., Khan, S., Kisielow, J., Klenerman, P., Knopf, J., Koay, H.F., Kobow, K., Kolls, J.K., Kong, W.T., Kopf, M., Korn, T., Kriegsmann, K., Kristyanto, H., Kroneis, T., Krueger, A., Kühne, J., Kukat, C., Kunkel, D., Kunze-Schumacher, H., Kurosaki, T., Kurts, C., Kvistborg, P., Kwok, I., Landry, J., Lantz, O., Lanuti, P., LaRosa, F., Lehuen, A., LeibundGut-Landmann, S., Leipold, M.D., Leung, L.Y.T., Levings, M.K., Lino, A.C., Liotta, F., Litwin, V., Liu, Y., Ljunggren, H.G., Lohoff, M., Lombardi, G., Lopez, L., López-Botet, M., Lovett-Racke, A.E., Lubberts, E., Luche, H., Ludewig, B., Lugli, E., Lunemann, S., Maecker, H.T., Maggi, L., Maguire, O., Mair, Fl., Mair, K.H., Mantovani, A., Manz, R.A., Marshall, A.J., Martínez-Romero, A., Martrus, G., Marventano, I., Maslinski, W., Matarese, G., Mattioli, A.V., Maueröder, C., Mazzoni, A., McCluskey, J., McGrath, M., McGuire, H.M., McInnes, I.B., Mei, H.E., Melchers, F., Melzer, S., Mielenz, D., Miller, S.D., Mills, K.H.G., Minderman, H., Mjösberg, J., Moore, J., Moran, B., Moretta, L., Mosmann, T.R., Müller, S., Multhoff, G., Muñoz, L.E., Münz, C., Nakayama, T., Nasi, M., Neumann, K., Ng, L.G., Niedobitek, A., Nourshargh, S., Núñez, G., O'Connor, J.E., Ochel, A., Oja, A., Ordonez, D., Orfao, A., Orlowski-Oliver, E., Ouyang, W., Oxenius, A., Palankar, R., Panse, I., Pattanapanyasat, K., Paulsen, M., Pavlinic, D., Penter, L., Peterson, P., Peth, C., Petriz, J., Piancone, F., Pickl, W.F., Piconese, S., Pinti, M., Pockley, A.G., Podolska, M.J., Poon, Z., Pracht, K., Prinz, I., Pucillo, C.E.M., Quataert, S.A., Quatrini, L., Quinn, K.M., Radbruch, H., Radstake, T.R.D.J., Rahmig, S., Rahn, H.P., Rajwa, B., Ravichandran, G., Raz, Y., Rebhahn, J.A., Recktenwald, D., Reimer, D., Reis E Sousa, C., Remmerswaal, E.B.M., Richter, L., Rico, L.G., Riddell, A., Rieger, A.M., Robinson, J.P, Romagnani, C., Rubartelli, A., Ruland, J., Saalmüller, A., Saeys, Y., Saito, T., Sakaguchi, S., Sala-de-Oyanguren, F., Samstag, Y., Sanderson, S., Sandrock, I., Santoni, A., Sanz, R.B., Saresella, M., Sautes-Fridman, C., Sawitzki, B., Schadt, L., Scheffold, A., Scherer, H.U, Schiemann, M., Schildberg, F.A., Schimisky, E., Schlitzer, A., Schlosser, J., Schmid, S., Schmitt, S., Schober, K., Schraivogel, D., Schuh, W., Schüler, T., Schulte, R., Schulz, A.R., Schulz, S.R., Scottá, C., Scott-Algara, D., Sester, D.P., Shankey, T.V., Silva-Santos, B., Simon, A.K., Sitnik, K.M., Sozzani, S., Speiser, D.E., Spidlen, J., Stahlberg, A., Stall, A.M., Stanley, N., Stark, R., Stehle, C., Steinmetz, T., Stockinger, H., Takahama, Y., Takeda, K., Tan, L., Tárnok, A., Tiegs, G., Toldi, G., Tornack, J., Traggiai, E., Trebak, M., Tree, T.I.M., Trotter, J., Trowsdale, J., Tsoumakidou, M., Ulrich, H., Urbanczyk, S., van de Veen, W., van den Broek, M., van der Pol, E., Van Gassen, S., Van Isterdael, G., van Lier, R.A.W., Veldhoen, M., Vento-Asturias, S., Vieira, P., Voehringer, D., Volk, H.D., von Borstel, A., von Volkmann, K., Waisman, A., Walker, R.V., Wallace, P.K., Wang, S.A., Wang, X.M., Ward, M.D., Ward-Hartstonge, K.A., Warnatz, K., Warnes, G., Warth, S., Waskow, C., Watson, J.V., Watzl, C., Wegener, L., Weisenburger, T., Wiedemann, A., Wienands, J., Wilharm, A., Wilkinson, R.J., Willimsky, G., Wing, J.B., Winkelmann, R., Winkler, T.H., Wirz, O.F., Wong, A., Wurst, P., Yang, J.H.M., Yang, J., Yazdanbakhsh, M., Yu, L., Yue, A., Zhang, H., Zhao, Y., Ziegler, S.M., Zielinski, C., Zimmermann, J. and Zychlinsky, A.
Abstract:	These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
Keywords:	Allergy and Immunology, Cell Separation, Consensus, Flow Cytometry, Phenotype
Source:	European Journal of Immunology
ISSN:	0014-2980
Publisher:	Wiley
Volume:	49
Number:	10
Page Range:	1457-1973
Date:	October 2019
Official Publication:	https://doi.org/10.1002/eji.201970107
External Fulltext:	View full text on PubMed Central
PubMed:	View item in PubMed

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