Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Efficient CRISPR/Cas9-mediated gene knockin in mouse hematopoietic stem and progenitor cells

[thumbnail of Original Article]
Preview
PDF (Original Article) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
5MB
[thumbnail of Supplement]
Preview
PDF (Supplement) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
9MB

Item Type:Article
Title:Efficient CRISPR/Cas9-mediated gene knockin in mouse hematopoietic stem and progenitor cells
Creators Name:Tran, N.T., Sommermann, T., Graf, R., Trombke, J., Pempe, J., Petsch, K., Kühn, R., Rajewsky, K. and Chu, V.T.
Abstract:Mutations accumulating in hematopoietic stem and progenitor cells (HSPCs) during development can cause severe hematological disorders. Modeling these mutations in mice is essential for understanding their functional consequences. Here, we describe an efficient CRISPR/Cas9-based system to knock in and repair genes in mouse HSPCs. CRISPR/Cas9 ribonucleoproteins, in combination with recombinant adeno-associated virus (rAAV)-DJ donor templates, led to gene knockin efficiencies of up to 30% in the Lmnb1 and Actb loci of mouse HSPCs in vitro. The targeted HSPCs engraft and reconstitute all immune cell lineages in the recipient mice. Using this approach, we corrected a neomycin-disrupted Rag2 gene. The Rag2-corrected HSPCs restore B and T cell development in vivo, confirming the functionality of the approach. Our method provides an efficient strategy to study gene function in the hematopoietic system and model hematological disorders in vivo, without the need for germline mutagenesis.
Keywords:CRISPR/Cas9, Ribonucleoprotein, RNP, Adeno-Associated Virus, AAV, Non-Homologous End Joining, NHEJ, Homologous Recombination, HR, Hematopoietic Stem and Progenitor Cells, HSPCs, High Efficiency, Gene Knockin, Gene Repair, Animals, Mice
Source:Cell Reports
ISSN:2211-1247
Publisher:Cell Press / Elsevier
Volume:28
Number:13
Page Range:3510-3522
Date:24 September 2019
Official Publication:https://doi.org/10.1016/j.celrep.2019.08.065
PubMed:View item in PubMed

Repository Staff Only: item control page

Downloads

Downloads per month over past year

Open Access
MDC Library