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Quantitative phase imaging-based concepts for the analysis of global morphology changes in confluent cell layers

Item Type:Conference or Workshop Item
Title:Quantitative phase imaging-based concepts for the analysis of global morphology changes in confluent cell layers
Creators Name:Kemper, B., Pohl, L., Kaiser, M., Doepker, E., Schnekenburger, J. and Ketelhut, S.
Abstract:We have explored strategies for the analysis of confluent cell layers utilizing histogram based-evaluation of quantitative phase images for example of digital holographic microscopy (DHM), a variant of quantitative phase microscopy (QPM). The applicability of the proposed numerical procedures is illustrated by the DHM-based quantification of drug induced cell morphology changes. The achieved results show that histogram-based evaluation of quantitative phase images allows a highly reliable detection and continuous observation of global cellular morphology changes in confluent cell layers.
Keywords:Quantitative Phase Imaging, Digital Holographic Microscopy, Label-Free Live Cell Imaging, Confluent Cell Layers
Source:Proceedings of SPIE
Series Name:Proceedings of SPIE
Title of Book:Quantitative Phase Imaging V
ISSN:0277-786X
Publisher:International Society for Optics and Photonics (SPIE)
Volume:10887
Page Range:108871M-1
Date:4 March 2019
Official Publication:https://doi.org/10.1117/12.2509242

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