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| Item Type: | Article |
|---|---|
| Title: | Chronic CD30-signaling in B cells results in lymphomagenesis by driving the expansion of plasmablasts and B1 cells |
| Creators Name: | Sperling, S.A., Fiedler, P., Lechner, M., Pollithy, A., Ehrenberg, S., Schiefer, A.I., Kenner, L., Feuchtinger, A., Kühn, R., Swinerd, G., Schmidt-Supprian, M., Strobl, L.J. and Zimber-Strobl, U. |
| Abstract: | CD30 is expressed on a variety of B-cell lymphomas such as Hodgkin's lymphoma, primary effusion lymphoma, and a subgroup of diffuse large B-cell lymphoma. In normal tissues, CD30 is expressed on some activated B and T lymphocytes. However, the physiological function of CD30-signaling and its contribution to the generation of CD30-positive lymphomas are still poorly understood. To gain a better understanding of CD30-signaling in B cells, we studied the expression of CD30 in different murine B-cell populations. We show that B1 cells express higher levels of CD30 than B2 cells and that CD30 is upregulated in IRF4(+) plasmablasts (PB). Furthermore, we generated and analyzed mice expressing a constitutively active CD30-receptor in B lymphocytes. These mice displayed an increase of B1 cells in the peritoneal cavity (PerC) and in secondary lymphoid organs as well as increased numbers of plasma cells (PC). TI-2-immunization resulted in a further expansion of B1 cells and PC. We provide evidence that the expanded B1 population in the spleen includes a fraction of PB. CD30 signals appeared to enhance PC-differentiation by increasing activation of NF-κB, higher levels of phosphorylated STAT3 and STAT6 and of nuclear IRF4. In addition, chronic CD30-signaling led to B-cell lymphomagenesis in aged mice. These lymphomas were localized in the spleen and PerC and had a B1-like/plasmablastic phenotype. We conclude that our mouse model mirrors chronic B cell activation with increased numbers of CD30(+) lymphocytes and provides experimental proof that chronic CD30-signaling increases the risk of B-cell lymphomagenesis. |
| Keywords: | B-Cell Lymphoma, B-Lymphocytes, B-Lymphoid Precursor Cells, Ki-1 Antigen, Neoplastic Cell Transformation, Plasma Cells, Signal Transduction, Transgenic Mice, Animals, Mice |
| Source: | Blood |
| ISSN: | 0006-4971 |
| Publisher: | American Society of Hematology |
| Volume: | 133 |
| Number: | 24 |
| Page Range: | 2597-2609 |
| Date: | 13 June 2019 |
| Additional Information: | Copyright © 2019 by The American Society of Hematology |
| Official Publication: | https://doi.org/10.1182/blood.2018880138 |
| PubMed: | View item in PubMed |
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