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| Item Type: | Article |
|---|---|
| Title: | The C/EBPβ LIP isoform rescues loss of C/EBPβ function in the mouse |
| Creators Name: | Bégay, V., Baumeier, C., Zimmermann, K., Heuser, A. and Leutz, A. |
| Abstract: | The transcription factor C/EBPβ regulates hematopoiesis, bone, liver, fat, and skin homeostasis, and female reproduction. C/EBPβ protein expression from its single transcript occurs by alternative in-frame translation initiation at consecutive start sites to generate three isoforms, two long (LAP*, LAP) and one truncated (LIP), with the same C-terminal bZip dimerization domain. The long C/EBPβ isoforms are considered gene activators, whereas the LIP isoform reportedly acts as a dominant-negative repressor. Here, we tested the putative repressor functions of the C/EBPβ LIP isoform in mice by comparing monoallelic WT or LIP knockin mice with Cebpb knockout mice, in combination with monoallelic Cebpa mice. The C/EBPβ LIP isoform was sufficient to function in coordination with C/EBPα in murine development, adipose tissue and sebocyte differentiation, and female fertility. Thus, the C/EBPβ LIP isoform likely has more physiological functions than its currently known role as a dominant-negative inhibitor, which are more complex than anticipated. |
| Keywords: | 3T3-L1 Cells, Adipose Tissue, Alleles, CCAAT-Enhancer-Binding Protein-beta, Fertility, Gene Knockout Techniques, Homeostasis, Phenotype, Protein Isoforms, Skin, Animals, Mice |
| Source: | Scientific Reports |
| ISSN: | 2045-2322 |
| Publisher: | Nature Publishing Group |
| Volume: | 8 |
| Number: | 1 |
| Page Range: | 8417 |
| Date: | 30 May 2018 |
| Official Publication: | https://doi.org/10.1038/s41598-018-26579-y |
| PubMed: | View item in PubMed |
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