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| Item Type: | Article |
|---|---|
| Title: | Transient cardiomyocyte fusion regulates cardiac development in zebrafish |
| Creators Name: | Sawamiphak, S., Kontarakis, Z., Filosa, A., Reischauer, S. and Stainier, D.Y.R. |
| Abstract: | Cells can sacrifice their individuality by fusing, but the prevalence and significance of this process are poorly understood. To approach these questions, here we generate transgenic reporter lines in zebrafish to label and specifically ablate fused cells. In addition to skeletal muscle cells, the reporters label cardiomyocytes starting at an early developmental stage. Genetic mosaics generated by cell transplantation show cardiomyocytes expressing both donor- and host-derived transgenes, confirming the occurrence of fusion in larval hearts. These fusion events are transient and do not generate multinucleated cardiomyocytes. Functionally, cardiomyocyte fusion correlates with their mitotic activity during development as well as during regeneration in adult animals. By analyzing the cell fusion-compromised jam3b mutants, we propose a role for membrane fusion in cardiomyocyte proliferation and cardiac function. Together, our findings uncover the previously unrecognized process of transient cardiomyocyte fusion and identify its potential role in cardiac development and function. |
| Keywords: | Cardiac Myocytes, Cell Differentiation, Cell Fusion, Cell Proliferation, Developmental Gene Expression Regulation, Genetically Modified Animals, Heart, Larva, Luminescent Proteins, Myocardium, Skeletal Muscle Fibers, Zebrafish Proteins, Animals, Zebrafish |
| Source: | Nature Communications |
| ISSN: | 2041-1723 |
| Publisher: | Nature Publishing Group |
| Volume: | 8 |
| Number: | 1 |
| Page Range: | 1525 |
| Date: | 15 November 2017 |
| Official Publication: | https://doi.org/10.1038/s41467-017-01555-8 |
| PubMed: | View item in PubMed |
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