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| Item Type: | Article |
|---|---|
| Title: | Quantitative GTPase affinity purification identifies Rho family protein interaction partners |
| Creators Name: | Paul, F., Zauber, H., von Berg, L., Rocks, O., Daumke, O. and Selbach, M. |
| Abstract: | Although Rho GTPases are essential molecular switches involved in many cellular processes, an unbiased experimental comparison of their interaction partners was not yet performed. Here, we develop quantitative GTPase affinity purification (qGAP) to systematically identify interaction partners of six Rho GTPases (Cdc42, Rac1, RhoA, RhoB, RhoC, RhoD) depending on their nucleotide loading state. The method works with cell line or tissue-derived protein lysates in combination with SILAC-based or label free quantification, respectively. We demonstrate that qGAP identifies known and novel binding partners that can be validated in an independent assay. Our interaction network for six Rho GTPases contains many novel binding partners, reveals highly promiscuous interaction of several effectors and mirrors evolutionary relationships among Rho GTPases. |
| Keywords: | Rho GTPase, Proteomics, Signaling, Protein-Protein Interaction, Animals, Mice |
| Source: | Molecular & Cellular Proteomics |
| ISSN: | 1535-9476 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Volume: | 16 |
| Number: | 1 |
| Page Range: | 73-85 |
| Date: | 1 January 2017 |
| Official Publication: | https://doi.org/10.1074/mcp.M116.061531 |
| PubMed: | View item in PubMed |
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