Acute physical exercise and long-term individual shear rate therapy increase telomerase activity in human peripheral blood mononuclear cells

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Item Type:	Article
Title:	Acute physical exercise and long-term individual shear rate therapy increase telomerase activity in human peripheral blood mononuclear cells
Creators Name:	Zietzer, A., Buschmann, E.E., Janke, D., Li, L., Brix, M., Meyborg, H., Stawowy, P., Jungk, C., Buschmann, I. and Hillmeister, P.
Abstract:	AIM: Physical activity is a potent way to impede vascular aging. However, patients who suffer from peripheral artery disease (PAD) are often unable to exercise adequately. For those patients, we have developed individual shear rate therapy (ISRT), which is an adaptation of external counterpulsation and enhances endovascular fluid shear stress to increase collateral growth (arteriogenesis). To evaluate the effects of physical exercise and ISRT on the telomere biology of peripheral blood mononuclear cells (PBMCs), we conducted two clinical trials. METHODS: In the ISRT-1 study, we assessed PBMC telomerase activity in 26 young healthy volunteers upon a single (short-term) ISRT session and a single treadmill running session. In the ISRT-2 study, we investigated PBMC telomere biology of 14 aged PAD patients, who underwent 30 hours of (long-term) ISRT within a 5-week-period. RESULTS: We demonstrate that telomerase activity significantly increased from 39.84 Total Product Generated Units (TPG) +/- 6.15 to 58.10 TPG +/- 10.46 upon a single treadmill running session in healthy volunteers. In the ISRT-2 trial, PBMC telomerase activity and the mRNA expression of the telomere-protective factor TRF2 increased from 40.87 TPG +/- 4.45 to 60.98 TPG +/- 6.83 and 2.21-fold +/- 0.48, respectively, upon long-term ISRT in aged PAD patients. CONCLUSION: In summary, we show that acute exercise and long-term ISRT counterpulsation positively affect PBMC telomerase activity, which is indicative for an improved regenerative potential of immune cells and vascular tissues. Long-term ISRT also enhances the gene expression of the telomere protective factor TRF2.
Keywords:	Peripheral Artery Disease, Telomerase, Leukocyte, Exercise, Arteriogenesis, Counterpulsation, Individual Shear Rate Therapy, Collateral Circulation
Source:	Acta Physiologica
ISSN:	1748-1708
Publisher:	Wiley
Volume:	220
Number:	5
Page Range:	251-262
Date:	June 2017
Official Publication:	https://doi.org/10.1111/apha.12820
PubMed:	View item in PubMed

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