In vivo interaction proteomics in Caenorhabditis elegans embryos provides new insights into P granule dynamics

Chen, J.X.; Cipriani, P.G.; Mecenas, D.; Polanowska, J.; Piano, F.; Gunsalus, K.C.; Selbach, M.

In vivo interaction proteomics in Caenorhabditis elegans embryos provides new insights into P granule dynamics

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Item Type:	Article
Title:	In vivo interaction proteomics in Caenorhabditis elegans embryos provides new insights into P granule dynamics
Creators Name:	Chen, J.X., Cipriani, P.G., Mecenas, D., Polanowska, J., Piano, F., Gunsalus, K.C. and Selbach, M.
Abstract:	Studying protein interactions in whole organisms is fundamental to understanding development. Here, we combine in vivo expressed GFP-tagged proteins with quantitative proteomics to identify protein-protein interactions of selected key proteins involved in early C. elegans embryogenesis. Co-affinity purification of interaction partners for eight bait proteins resulted in a pilot in vivo interaction map of proteins with a focus on early development. Our network reflects known biology and is highly enriched in functionally relevant interactions. To demonstrate the utility of the map, we looked for new regulators of P granule dynamics and found that GEI-12, a novel binding partner of the DYRK family kinase MBK-2, is a key regulator of P granule formation and germline maintenance. Our data corroborate a recently proposed model in which the phosphorylation state of GEI-12 controls P granule dynamics. In addition, we find that GEI-12 also induces granule formation in mammalian cells, suggesting a common regulatory mechanism in worms and humans. Our results show that in vivo interaction proteomics provides unique insights into animal development.
Keywords:	Affinity Chromatography, Caenorhabditis elegans Proteins, Carrier Proteins, Cytoplasmic Granules, Developmental Gene Expression Regulation, Mass Spectrometry, Phosphorylation, Protein Interaction Maps, Protein-Serine-Threonine Kinases, Protein-Tyrosine Kinases, Proteomics, Animals, Caenorhabditis elegans
Source:	Molecular & Cellular Proteomics
ISSN:	1535-9476
Publisher:	American Society for Biochemistry and Molecular Biology
Volume:	15
Number:	5
Page Range:	1642-1657
Date:	1 May 2016
Official Publication:	https://doi.org/10.1074/mcp.M115.053975
PubMed:	View item in PubMed

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