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| Item Type: | Article |
|---|---|
| Title: | In vivo interaction proteomics in Caenorhabditis elegans embryos provides new insights into P granule dynamics |
| Creators Name: | Chen, J.X., Cipriani, P.G., Mecenas, D., Polanowska, J., Piano, F., Gunsalus, K.C. and Selbach, M. |
| Abstract: | Studying protein interactions in whole organisms is fundamental to understanding development. Here, we combine in vivo expressed GFP-tagged proteins with quantitative proteomics to identify protein-protein interactions of selected key proteins involved in early C. elegans embryogenesis. Co-affinity purification of interaction partners for eight bait proteins resulted in a pilot in vivo interaction map of proteins with a focus on early development. Our network reflects known biology and is highly enriched in functionally relevant interactions. To demonstrate the utility of the map, we looked for new regulators of P granule dynamics and found that GEI-12, a novel binding partner of the DYRK family kinase MBK-2, is a key regulator of P granule formation and germline maintenance. Our data corroborate a recently proposed model in which the phosphorylation state of GEI-12 controls P granule dynamics. In addition, we find that GEI-12 also induces granule formation in mammalian cells, suggesting a common regulatory mechanism in worms and humans. Our results show that in vivo interaction proteomics provides unique insights into animal development. |
| Keywords: | Affinity Chromatography, Caenorhabditis elegans Proteins, Carrier Proteins, Cytoplasmic Granules, Developmental Gene Expression Regulation, Mass Spectrometry, Phosphorylation, Protein Interaction Maps, Protein-Serine-Threonine Kinases, Protein-Tyrosine Kinases, Proteomics, Animals, Caenorhabditis elegans |
| Source: | Molecular & Cellular Proteomics |
| ISSN: | 1535-9476 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Volume: | 15 |
| Number: | 5 |
| Page Range: | 1642-1657 |
| Date: | 1 May 2016 |
| Official Publication: | https://doi.org/10.1074/mcp.M115.053975 |
| PubMed: | View item in PubMed |
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