Genome editing in mice using TALE nucleases

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Item Type:	Article
Title:	Genome editing in mice using TALE nucleases
Creators Name:	Wefers, B., Brandl, C., Ortiz, O., Wurst, W. and Kühn, R.
Abstract:	Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as sequence-specific nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step without the need for embryonic stem cells. By embryo microinjection of TALEN mRNAs and targeting vectors, knockout and knock-in alleles can be generated fast and efficiently. In this chapter we provide protocols for the application of TALENs in mouse zygotes.
Keywords:	Knock-in, Knockout, One-cell Embryo, Gene Targeting, TALEN, Mouse, Animals, Mice
Source:	Methods in Molecular Biology
Series Name:	Methods in Molecular Biology
Title of Book:	TALENs : methods and protocols
ISSN:	1064-3745
ISBN:	978-1-4939-2931-3
Publisher:	Springer / Humana Press
Volume:	1338
Page Range:	229-243
Number of Pages:	274
Date:	2016
Official Publication:	https://doi.org/10.1007/978-1-4939-2932-0_17
PubMed:	View item in PubMed

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