Imaging protein interactions by FRET microscopy: labeling proteins with fluorescent dyes

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Item Type:	Article
Title:	Imaging protein interactions by FRET microscopy: labeling proteins with fluorescent dyes
Creators Name:	Verveer, P.J., Rocks, O., Harpur, A.G. and Bastiaens, P.I.H.
Abstract:	This protocol provides a method for labeling proteins, such as antibodies and purified recombinant proteins, with succinimide esters of sulfoindocyanine (Cy) dyes. Cy dyes covalently bind to free amino groups (the {alpha}-amino-terminal or {epsilon}-amino groups on lysine side chains).
Source:	Cold Spring Harbor Protocols
ISSN:	1559-6095
Publisher:	Cold Spring Harbor Laboratory Press
Volume:	2006
Number:	6
Date:	1 November 2006
Official Publication:	https://doi.org/10.1101/pdb.prot4645
PubMed:	View item in PubMed

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