Imaging protein interactions by FRET microscopy: cell preparation for FRET analysis

Tools

Item Type:	Article
Title:	Imaging protein interactions by FRET microscopy: cell preparation for FRET analysis
Creators Name:	Verveer, P.J., Rocks, O., Harpur, A.G. and Bastiaens, P.I.H.
Abstract:	The following protocol describes the preparation of cells for FRET analysis on live and fixed cells. The reagents used have been optimized to minimize the quenching of GFP mutants and fluorescent dyes.
Source:	Cold Spring Harbor Protocols
ISSN:	1559-6095
Publisher:	Cold Spring Harbor Laboratory Press
Volume:	2006
Number:	6
Date:	1 November 2006
Official Publication:	https://doi.org/10.1101/pdb.prot4646
PubMed:	View item in PubMed

Repository Staff Only: item control page