Analysis of impedance-based cellular growth assays

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Item Type:	Article
Title:	Analysis of impedance-based cellular growth assays
Creators Name:	Witzel, F., Fritsche-Guenther, R., Lehmann, N., Sieber, A. and Blüthgen, N.
Abstract:	MOTIVATION: Impedance-based technologies are advancing methods for measuring proliferation of adherent cell cultures non-invasively and in real time. The analysis of the resulting data has so far been hampered by inappropriate computational methods and the lack of systematic data to evaluate the characteristics of the assay. RESULTS: We used a commercially available system for impedance-based growth measurement (xCELLigence), and compared the reported cell index with data from microscopy. We found that the measured signal correlates linearly with the cell number throughout the time of an experiment with sufficient accuracy in subconfluent cell cultures. The resulting growth curves for various colon cancer cells could be well described with the empirical Richards growth model, which allows for extracting quantitative parameters (such as characteristic cycle times). We found that frequently used readouts like the cell index at a specific time or the area under the growth curve cannot be used to faithfully characterize growth inhibition. We propose to calculate the average growth rate of selected time intervals to accurately estimate time-dependent IC50-values of drugs from growth curves.
Keywords:	Biological Assay, Biological Models, Cell Adhesion, Cell Cycle, Cell Proliferation, Cells, Electric Impedance, Inhibitory Concentration 50, Small Molecule Libraries, Time Factors, Tumor Cell Line
Source:	Bioinformatics
ISSN:	1367-4803
Publisher:	Oxford University Press
Volume:	31
Number:	16
Page Range:	2705-2712
Date:	15 August 2015
Official Publication:	https://doi.org/10.1093/bioinformatics/btv216
PubMed:	View item in PubMed

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