A method for the generation of TCR retrogenic mice

Tools

Item Type:	Article
Title:	A method for the generation of TCR retrogenic mice
Creators Name:	Kieback, E., Hilgenberg, E. and Fillatreau, S.
Abstract:	Retrogenic mice provide a unique system for rapidly analyzing the function of genes in the hematopoietic system. Here, we provide a detailed protocol for the production of retrogenic mice expressing genes coding for T cell receptor (TCR) for antigen. This technology should be easy to establish in any laboratory and should allow for a rapid progress in our understanding of the functional roles of TCR repertoires in immunity.
Keywords:	T Cell Receptor for Antigen (TCR), Retrogenic Mice, Retrovirus, Transduction, Transfection, Animals, Mice
Source:	Methods in Molecular Biology
Series Name:	Methods in Molecular Biology
Title of Book:	T-helper cells : methods and protocols
ISSN:	1064-3745
ISBN:	978-1-4939-1211-7
Publisher:	Springer / Humana Press
Volume:	1193
Page Range:	117-126
Date:	2014
Official Publication:	https://doi.org/10.1007/978-1-4939-1212-4_12
PubMed:	View item in PubMed

Repository Staff Only: item control page