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| Item Type: | Article |
|---|---|
| Title: | A B cell-deficient mouse by targeted disruption of the membrane exon of the immunoglobulin μ chain gene |
| Creators Name: | Kitamura, D., Roes, J., Kühn, R. and Rajewsky, K. |
| Abstract: | Of the various classes of antibodies that B lymphocytes can produce, class M (IgM) is the first to be expressed on the membrane of the developing cells. Pre-B cells, the precursors of B-lymphocytes, produce the heavy chain of IgM (μ chain), but not light chains. Recent data suggest that pre-B cells express mu chains on the membrane together with the 'surrogate' light chains lambda 5 and V pre B (refs 2-7). This complex could control pre-B-cell differentiation, in particular the rearrangement of the light-chain genes. We have now assessed the importance of the membrane form of the μ chain in B-cell development by generating mice lacking this chain. We disrupted one of the membrane exons of the gene encoding the μ-chain constant region by gene targeting in mouse embryonic stem cells. From these cells we derived mice heterozygous or homozygous for the mutation. B-cell development in the heterozygous mice seemed to be normal, but in homozygous animals B cells were absent, their development already being arrested at the stage of pre-B-cell maturation. |
| Keywords: | B-Cell Antigen Receptors, B-Lymphocyte Differentiation Antigens, B-Lymphocytes, DNA Mutational Analysis, Flow Cytometry, Hematopoiesis, Immunoglobulin Genes, Immunoglobulin Light Chains, Immunoglobulin mu-Chains, Immunologic Deficiency Syndromes, Membrane Glycoproteins, Southern Blotting, Surrogate Immunoglobulin Light Chains, Animals, Mice |
| Source: | Nature |
| ISSN: | 0028-0836 |
| Publisher: | Nature Publishing Group |
| Volume: | 350 |
| Number: | 6317 |
| Page Range: | 423-426 |
| Date: | 4 April 1991 |
| Official Publication: | https://doi.org/10.1038/350423a0 |
| PubMed: | View item in PubMed |
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