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| Item Type: | Article |
|---|---|
| Title: | Temporally and spatially regulated somatic mutagenesis in mice |
| Creators Name: | Schwenk, F., Kühn, R., Angrand, P.O., Rajewsky, K. and Stewart, A.F. |
| Abstract: | In mice transgenesis through oocyte injection or DNA recombination in embryonal stem (ES) cells allows mutations to be introduced into the germline. However, the earliest phenotype of the introduced mutation can eclipse later effects. We show in mice that site-specific genomic recombination can be induced in a selected cell type, B lymphocytes, at a chosen time. This precision of somatic mutagenesis was accomplished by limiting expression of a Cre recombinase-estrogen receptor fusion protein to B lymphocytes by use of tissue-specific elements in the promoter of the transgene employed. The expressed fusion protein remained inactive until derepressed by systemic administration of an exogenous ligand for the estrogen receptor, 4-OH-tamoxifen. Upon derepression the Cre recombinase enzyme deleted specific DNA segments, flanked by loxP sites, in B lymphocytes only. The efficiency of recombination in cells expressing the fusion protein could be varied from low levels to >80%, depending on the dose of ligand administered. Our work presents a paradigm applicable to other uses of site-specific recombination in somatic mutagenesis where both temporal and spatial regulation are desired. |
| Keywords: | B-Lymphocytes, Cell Line, Estrogen Receptors, Gene Deletion, Gene Expression, Genetic Recombination, Integrases, Ligands, Mutagenesis, Recombinant Fusion Proteins, Tamoxifen, Viral Proteins, Animals, Mice |
| Source: | Nucleic Acids Research |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Volume: | 26 |
| Number: | 6 |
| Page Range: | 1427-1432 |
| Date: | 15 March 1998 |
| Official Publication: | https://doi.org/10.1093/nar/26.6.1427 |
| PubMed: | View item in PubMed |
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