Measuring the size of biological nanostructures with spatially modulated illumination microscopy

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Item Type:	Article
Title:	Measuring the size of biological nanostructures with spatially modulated illumination microscopy
Creators Name:	Martin, S., Failla, A.V., Spoeri, U., Cremer, C. and Pombo, A.
Abstract:	Spatially modulated illumination fluorescence microscopy can in theory measure the sizes of objects with a diameter ranging between 10 and 200 nm and has allowed accurate size measurement of subresolution fluorescent beads ( approximately 40-100 nm). Biological structures in this size range have so far been measured by electron microscopy. Here, we have labeled sites containing the active, hyperphosphorylated form of RNA polymerase II in the nucleus of HeLa cells by using the antibody H5. The spatially modulated illumination-microscope was compared with confocal laser scanning and electron microscopes and found to be suitable for measuring the size of cellular nanostructures in a biological setting. The hyperphosphorylated form of polymerase II was found in structures with a diameter of approximately 70 nm, well below the 200-nm resolution limit of standard fluorescence microscopes.
Keywords:	Cell Nucleus Structures, Cell Nucleus, Fluorescence Microscopy, Fluorescent Antibody Technique, HeLa Cells, Microscopy, Nanotechnology, RNA Polymerase II
Source:	Molecular Biology of the Cell
ISSN:	1059-1524
Publisher:	American Society for Cell Biology
Volume:	15
Number:	5
Page Range:	2449-2455
Date:	May 2004
Official Publication:	https://doi.org/10.1091/mbc.E04-01-0045
PubMed:	View item in PubMed

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