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| Item Type: | Article |
|---|---|
| Title: | Utilization of TREC and KREC quantification for the monitoring of early T- and B-cell neogenesis in adult patients after allogeneic hematopoietic stem cell transplantation |
| Creators Name: | Mensen, A., Ochs, C., Stroux, A., Wittenbecher, F., Szyska, M., Imberti, L., Fillatreau, S., Uharek, L., Arnold, R., Dörken, B., Thiel, A., Scheibenbogen, C. and Na, I.K. |
| Abstract: | BACKGROUND: After hematopoietic stem cell transplantation (HSCT) T- and B-cell reconstitution from primary lymphoid organs are a prerequisite for an effective early lymphocyte reconstitution and a long-term survival for adult patients suffering from acute leukemia. Here, we asked whether quantification of T cell receptor excision circle, (TREC) and kappa-deleting recombination excision circle (KREC) before and within six month after allogeneic HSCT could be used to measure the thymic and bone marrow outputs in such patients. METHODS: We used a duplex real time PCR assay to quantify the absolute copy counts of TREC and KREC, and correlated the data with absolute cell counts of CD3+CD4+ T-cell and CD19+ B-cell subsets determined by flow cytometry, respectively. RESULTS: By comparing two recently proposed naive T cell subsets, CD31+ naive and CD31- naive T cells, we found a better correlation for the CD31+ subset with TREC level post alloHSCT, in line with the assumption that it contained T cells recently derived from the thymus, indicating that TREC levels reflected real thymic de novo production. Transitional as well as naive B cells highly correlated with KREC levels, which suggested an association of KREC levels with ongoing bone marrow B cell output. CD45RO+ memory T cells and CD27+ memory B cells were significantly less correlated with TREC and KREC recovery, respectively. CONCLUSION: We conclude that simultaneous TREC/ KREC quantification is as a suitable and practicable method to monitor thymic and bone marrow output post alloHSCT in adult patients diagnosed with acute leukemia. |
| Keywords: | Allogeneic Hematopoietic Stem Cell Transplantation, Acute Leukemia, Simultaneous TREC/KREC Quantification Assay, Monitoring Immune Reconstitution |
| Source: | Journal of Translational Medicine |
| ISSN: | 1479-5876 |
| Publisher: | BioMed Central |
| Volume: | 11 |
| Number: | 1 |
| Page Range: | 188 |
| Date: | 14 August 2013 |
| Official Publication: | https://doi.org/10.1186/1479-5876-11-188 |
| PubMed: | View item in PubMed |
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