The ZFP-1(AF10)/DOT-1 complex opposes H2B ubiquitination to reduce Pol II transcription

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Item Type:	Article
Title:	The ZFP-1(AF10)/DOT-1 complex opposes H2B ubiquitination to reduce Pol II transcription
Creators Name:	Cecere, G., Hoersch, S., Jensen, M.B., Dixit, S. and Grishok, A.
Abstract:	The inhibition of transcriptional elongation plays an important role in gene regulation in metazoans, including C. elegans. Here, we combine genomic and biochemical approaches to dissect a role of ZFP-1, the C. elegans AF10 homolog, in transcriptional control. We show that ZFP-1 and its interacting partner DOT-1.1 have a global role in negatively modulating the level of polymerase II (Pol II) transcription on essential widely expressed genes. Moreover, the ZFP-1/DOT-1.1 complex contributes to progressive Pol II pausing on essential genes during development and to rapid Pol II pausing during stress response. The slowing down of Pol II transcription by ZFP-1/DOT-1.1 is associated with an increase in H3K79 methylation and a decrease in H2B monoubiquitination, which promotes transcription. We propose a model wherein the recruitment of ZFP-1/DOT-1.1 and deposition of H3K79 methylation at highly expressed genes initiates a negative feedback mechanism for the modulation of their expression.
Keywords:	Caenorhabditis Elegans, Caenorhabditis Elegans Proteins, Chromatin Immunoprecipitation, DNA Polymerase II, Gene Expression Regulation, Gene Knockdown Techniques, Genetic Promoter Regions, Genetic Transcription, Heat-Shock Response, Helminth Genes, Histone-Lysine N-Methyltransferase, Histones, Protein Transport, RNA Interference, Transcription Factors, Ubiquitination, Animals
Source:	Molecular Cell
ISSN:	1097-2765
Publisher:	Cell Press
Volume:	50
Number:	6
Page Range:	894-907
Date:	27 June 2013
Official Publication:	https://doi.org/10.1016/j.molcel.2013.06.002
PubMed:	View item in PubMed

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