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| Item Type: | Article |
|---|---|
| Title: | The first aptamer-apheresis column specifically for clearing blood of beta1-receptor autoantibodies : a successful proof of principle using autoantibody-positive SHR rats |
| Creators Name: | Wallukat, G., Haberland, A., Berg, S., Schulz, A., Freyse, E.J., Dahmen, C., Kage, A., Dandel, M., Vetter, R., Salzsieder, E., Kreutz, R. and Schimke, I. |
| Abstract: | Background: Application of immunoapheresis to eliminate pathogenic autoantibodies targeting the second extracellular loop of the beta1-receptor (beta1-AABs) is currently investigated in patients with cardiomyopathy. Aptamers (single short DNA or RNA strands) are a new class of molecules that bind to a specific target molecule. This property qualifies aptamers for potential use in the apheresis technique. We recently identified an aptamer that specifically binds to beta1-AABs, so in the present study we tested whether this aptamer could be used as a binder to prepare an apheresis column suitable for clearing beta1-AABs from rat's blood. Methods and Results: An apheresis column was designed containing the beta1-AAB-targeting-aptamer coupled to sepharose. As tested in vitro, this column (1) binds beta1-AABs highly specifically without marked interference with common IgGs, (2) has a capacity for clearing of approximately 1L of beta1-AAB-positive serum and (3) can be completely regenerated for subsequent use. Using the column for extracorporeal apheresis of spontaneously hypertensive rats (SHR) positive for both beta1-AABs and muscarinic 2-receptor autoantibodies (M2-AABs), only beta1-AABs were removed. In a follow-up of 9 weeks, recurrence of beta1-AABs in the blood of SHR could not be detected. Conclusions: For the first time, a newly designed apheresis column with a beta1-AAB specific aptamer as a binder was successfully used to eliminate beta1-AABs from SHR blood. |
| Keywords: | Apheresis, Aptamer, Autoantibody, Dilated Cardiomyopathy, Immunoadsorption, Animals, Rabbits, Rats |
| Source: | Circulation Journal |
| ISSN: | 1346-9843 |
| Publisher: | Japanese Circulation Society |
| Volume: | 76 |
| Number: | 10 |
| Page Range: | 2449-2455 |
| Date: | 25 September 2012 |
| Official Publication: | https://doi.org/10.1253/circj.CJ-12-0212 |
| PubMed: | View item in PubMed |
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