Regulation of microRNA expression and abundance during lymphopoiesis

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Item Type:	Article
Title:	Regulation of microRNA expression and abundance during lymphopoiesis
Creators Name:	Kuchen, S., Resch, W., Yamane, A., Kuo, N., Li, Z., Chakraborty, T., Wei, L., Laurence, A., Yasuda, T., Peng, S., Hu-Li, J., Lu, K., Dubois, W., Kitamura, Y., Charles, N., Sun, H.W., Muljo, S., Schwartzberg, P.L., Paul, W.E., O'Shea, J., Rajewsky, K. and Casellas, R.
Abstract:	Although the cellular concentration of miRNAs is critical to their function, how miRNA expression and abundance are regulated during ontogeny is unclear. We applied miRNA-, mRNA-, and ChIP-Seq to characterize the microRNome during lymphopoiesis within the context of the transcriptome and epigenome. We show that lymphocyte-specific miRNAs are either tightly controlled by polycomb group-mediated H3K27me3 or maintained in a semi-activated epigenetic state prior to full expression. Because of miRNA biogenesis, the cellular concentration of mature miRNAs does not typically reflect transcriptional changes. However, we uncover a subset of miRNAs for which abundance is dictated by miRNA gene expression. We confirm that concentration of 5p and 3p miRNA strands depends largely on free energy properties of miRNA duplexes. Unexpectedly, we also find that miRNA strand accumulation can be developmentally regulated. Our data provide a comprehensive map of immunity's microRNome and reveal the underlying epigenetic and transcriptional forces that shape miRNA homeostasis.
Keywords:	Genetic Epigenesis, Gene Expression, Gene Expression Regulation, Lymphocytes, Lymphopoiesis, MicroRNAs, Reverse Transcriptase Polymerase Chain Reaction, Animals, Mice
Source:	Immunity
ISSN:	1074-7613
Publisher:	Cell Press / Elsevier
Volume:	32
Number:	6
Page Range:	828-839
Date:	25 June 2010
Official Publication:	https://doi.org/10.1016/j.immuni.2010.05.009
PubMed:	View item in PubMed

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