![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
![[thumbnail of 11152oa.pdf]](https://edoc.mdc-berlin.de/style/images/fileicons/application_pdf.png) |
PDF
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
1MB |
| Item Type: | Article |
|---|---|
| Title: | Comprehensive survey of human brain microRNA by deep sequencing |
| Creators Name: | Shao, N.Y., Hu, H.Y., Yan, Z., Xu, Y., Hu, H., Menzel, C., Li, N., Chen, W. and Khaitovich, P. |
| Abstract: | BACKGROUND: MicroRNA (miRNA) play an important role in gene expression regulation. At present, the number of annotated miRNA continues to grow rapidly, in part due to advances of high-throughput sequencing techniques. Here, we use deep sequencing to characterize a population of small RNA expressed in human and rhesus macaques brain cortex. RESULTS: Based on a total of more than 150 million sequence reads we identify 197 putative novel miRNA, in humans and rhesus macaques, that are highly conserved among mammals. These putative miRNA have significant excess of conserved target sites in genes' 3'UTRs, supporting their functional role in gene regulation. Additionally, in humans and rhesus macaques respectively, we identify 41 and 22 conserved putative miRNA originating from non-coding RNA (ncRNA) transcripts. While some of these molecules might function as conventional miRNA, others might be harmful and result in target avoidance. CONCLUSIONS: Here, we further extend the repertoire of conserved human and rhesus macaque miRNA. Even though our study is based on a single tissue, the coverage depth of our study allows identification of functional miRNA present in brain tissue at background expression levels. Therefore, our study might cover large proportion of the yet unannotated conserved miRNA present in the human genome. |
| Keywords: | Base Sequence, Brain, Conserved Sequence, Gene Expression Profiling, MicroRNAs, Prefrontal Cortex, Untranslated RNA, DNA Sequence Analysis, Animals, Macaca mulatta |
| Source: | BMC Genomics |
| ISSN: | 1471-2164 |
| Publisher: | BioMed Central |
| Volume: | 11 |
| Number: | 1 |
| Page Range: | 409 |
| Date: | 30 June 2010 |
| Official Publication: | https://doi.org/10.1186/1471-2164-11-409 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
