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| Item Type: | Article |
|---|---|
| Title: | Commensal microflora and interferon-gamma promote steady-state interleukin-7 production in vivo |
| Creators Name: | Shalapour, S., Deiser, K., Sercan, O., Tuckermann, J., Minnich, K., Willimsky, G., Blankenstein, T., Haemmerling, G.J., Arnold, B. and Schueler, T. |
| Abstract: | IL-7 is a major regulator of lymphocyte homeostasis; however, little is known about the mechanisms that regulate IL-7 production. To study Il7 gene regulation in vivo, we generated a novel IL-7-reporter mouse, which allows the non-invasive quantification of Il7 gene activity in live mice and, additionally, the simultaneous activation/inactivation of target genes in IL-7-producing cells. With these IL-7-reporter mice, we identify thymus, skin and intestine as major sources of IL-7 in vivo. Importantly, we show that IFN-gamma and the commensal microflora promote steady-state IL-7 production in the intestine. Furthermore, we demonstrate that the blockade of IFN-gamma signaling in intestinal epithelial cells strongly reduces their IFN-gamma-driven IL-7 production. In summary, our data suggest a feedback loop in which commensal bacteria drive IFN-gamma production by lymphocytes, which in turn promotes epithelial cell IL-7 production and the survival of IL-7-dependent lymphocytes. |
| Keywords: | IL-7, IFN-gamma, Intestinal Epithelial Cells, Commensal Microflora, Animals, Mice |
| Source: | European Journal of Immunology |
| ISSN: | 0014-2980 |
| Publisher: | Wiley |
| Volume: | 40 |
| Number: | 9 |
| Page Range: | 2391-2400 |
| Date: | September 2010 |
| Official Publication: | https://doi.org/10.1002/eji.201040441 |
| PubMed: | View item in PubMed |
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