YB-1 relocates to the nucleus in adenovirus-infected cells and facilitates viral replication by inducing E2 gene expression through the E2 late promoter

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Item Type:	Article
Title:	YB-1 relocates to the nucleus in adenovirus-infected cells and facilitates viral replication by inducing E2 gene expression through the E2 late promoter
Creators Name:	Holm, P.S., Bergmann, S., Juerchott, K., Lage, H., Brand, K., Ladhoff, A., Mantwill, K., Curiel, D.T., Dobbelstein, M., Dietel, M., Gaensbacher, B. and Royer, H.D.
Abstract:	The adenovirus early proteins E1A and E1B-55kDa are key regulators of viral DNA replication, and it was thought that targeting of p53 by E1B-55kDa is essential for this process. Here we have identified a previously unrecognized function of E1B for adenovirus replication. We found that E1B-55kDa is involved in targeting the transcription factor YB-1 to the nuclei of adenovirus type 5-infected cells where it is associated with viral inclusion bodies believed to be sites of viral transcription and replication. We show that YB-1 facilitates E2 gene expression through the E2 late promoter thus controlling E2 gene activity at later stages of infection. The role of YB-1 for adenovirus replication was demonstrated with an E1-minus adenovirus vector containing a YB-1 transgene. In infected cells, AdYB-1 efficiently replicated and produced infectious progeny particles. Thus, adenovirus E1B-55kDa protein and the host cell factor YB-1 act jointly to facilitate adenovirus replication in the late phase of infection.
Keywords:	Adenoviridae, Adenovirus E2 Proteins, Bacterial Antigens, Bacterial Proteins, CCAAT-Enhancer-Binding Proteins, Cell Line, Cell Nucleus, Cell Nucleus Active Transport, Complementary DNA, Confocal Microscopy, DNA-Binding Proteins, Electron Microscopy, Fluorescence Microscopy, Gene Expression Regulation, Hela Cells, NFI Transcription Factors, Northern Blotting, Promoter Regions, Protein Binding, Southern Blotting, Transcription Factors, Transgenes, Virus Replication, Western Blotting, Y-Box-Binding Protein 1
Source:	Journal of Biological Chemistry
ISSN:	0021-9258
Publisher:	American Society for Biochemistry and Molecular Biology
Volume:	277
Number:	12
Page Range:	10427-10434
Date:	1 January 2002
Official Publication:	http://www.jbc.org/cgi/content/abstract/M106955200v1
PubMed:	View item in PubMed

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