Impact of genome reduction on bacterial metabolism and its regulation

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Item Type:	Article
Title:	Impact of genome reduction on bacterial metabolism and its regulation
Creators Name:	Yus, E., Maier, T., Michalodimitrakis, K., van Noort, V., Yamada, T., Chen, W.H., Wodke, J.A., Gueell, M., Martinez, S., Bourgeois, R., Kuehner, S., Raineri, E., Letunic, I., Kalinina, O.V., Rode, M., Herrmann, R., Gutierrez-Gallego, R., Russell, R.B., Gavin, A.C., Bork, P. and Serrano, L.
Abstract:	To understand basic principles of bacterial metabolism organization and regulation, but also the impact of genome size, we systematically studied one of the smallest bacteria, Mycoplasma pneumoniae. A manually curated metabolic network of 189 reactions catalyzed by 129 enzymes allowed the design of a defined, minimal medium with 19 essential nutrients. More than 1300 growth curves were recorded in the presence of various nutrient concentrations. Measurements of biomass indicators, metabolites, and (13)C-glucose experiments provided information on directionality, fluxes, and energetics; integration with transcription profiling enabled the global analysis of metabolic regulation. Compared with more complex bacteria, the M. pneumoniae metabolic network has a more linear topology and contains a higher fraction of multifunctional enzymes; general features such as metabolite concentrations, cellular energetics, adaptability, and global gene expression responses are similar, however.
Keywords:	Adenosine Triphosphate, Bacterial Proteins, Culture Media, Energy Metabolism, Enzymes, Gene Expression Profiling, Bacterial Gene Expression Regulation, Bacterial Genome, Glycolysis, Metabolic Networks and Pathways, Mycoplasma pneumoniae, Bacterial RNA, Signal Transduction, Systems Biology, Genetic Transcription, rRNA Operon
Source:	Science
ISSN:	0036-8075
Publisher:	American Association for the Advancement of Science
Volume:	326
Number:	5957
Page Range:	1263-1268
Date:	27 November 2009
Official Publication:	https://doi.org/10.1126/science.1177263
PubMed:	View item in PubMed

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