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| Item Type: | Article |
|---|---|
| Title: | The cortical microenvironment drives early immune organization and controls early osteoclastogenesis in bone healing |
| Creators: |
Noom, Anne  ORCID: https://orcid.org/0009-0006-8589-5350, Oktay, Hülya Zeynep, Morgan, Duncan M. ORCID: https://orcid.org/0000-0002-3253-1362, Kroh, Sandy, Kasapi, Ana, Ellinghaus, Agnes, Kochan, Merle, Bolaji, Olufemi, Uecker, Ralf, Günther, Robert, Bianchi, M. Paula, Bucher, Christian H., Hildebrandt, Alexander ORCID: https://orcid.org/0000-0003-3560-1642, Haas, Simon ORCID: https://orcid.org/0000-0001-9227-2051, Schmidt-Bleek, Katharina ORCID: https://orcid.org/0000-0002-5719-0867, Triantafyllopoulou, Antigoni, Hauser, Anja E. ORCID: https://orcid.org/0000-0002-7725-9526, Sawitzki, Birgit ORCID: https://orcid.org/0000-0001-8166-8579 and Duda, Georg N. ORCID: https://orcid.org/0000-0001-7605-3908
|
| Abstract: | Bone regeneration is a complex, tightly regulated process involving coordinated interactions of immune and stromal cells. Early phases of healing rely on the timely clearance of debris, a task primarily carried out by macrophages and osteoclasts. However, the sequence of events leading to the presence of osteoclasts at the fracture site and how this is shaped by local tissue microenvironments remains poorly understood, particularly at single-cell and spatial resolution. Using single-cell RNA sequencing and multi-epitope ligand cartography, we mapped the spatial organization of distinct cell compartments engaged in early fracture healing in both young and aged female mice at the start of healing. Surprisingly, we found that young mice exhibited an increased presence of activated osteoclasts at day 7, concentrated within the cortical niche. This compartment was also characterized by a spatially restricted immune response with a selective accumulation of distinct macrophage types jointly interacting with neutrophils and stromal cells. This raised the possibility that local cell organization influences osteoclast precursor differentiation. We identified a fracture-associated Spp1(hi) macrophage subset enriched at the cortex, which represented a transitional monocyte-derived state that expressed early osteoclast differentiation transcripts and gave rise to osteoclasts ex vivo. Neutrophils preceded fracture-associated Spp1(hi) macrophage accumulation and may promote their recruitment through chemotactic signaling. This coordination was less pronounced in aged mice despite preserved transcriptional states. In parallel, stromal cells in young animals displayed higher expression of essential niche factors, further supporting local osteoclastogenesis at the cortex. Together, our findings identify a distinct macrophage state that contains cells with osteoclast differentiation potential and reveal early, cortex-specific niche activity supporting osteoclastogenesis. This provides a new framework for understanding the initiation of spatial immune-stromal interactions for the early stages of regeneration. |
| Keywords: | Bone Regeneration, Cell Differentiation, Cellular Microenvironment, Cortical Bone, Fracture Healing, Inbred C57BL Mice, Macrophages, Neutrophils, Osteoclasts, Osteogenesis, Stromal Cells, Animals, Mice |
| Source: | Nature Communications |
| ISSN: | 2041-1723 |
| Publisher: | Nature Publishing Group |
| Volume: | 17 |
| Number: | 1 |
| Page Range: | 9154 |
| Date: | 27 August 2026 |
| Official Publication: | https://doi.org/10.1038/s41467-026-77196-7 |
| PubMed: | View item in PubMed |
| Related to: |
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