![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
Preview |
PDF (Original Article)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
4MB |
![[thumbnail of Supplementary Information incl. Source Data]](https://edoc.mdc-berlin.de/style/images/fileicons/other.png) |
Other (Supplementary Information incl. Source Data)
33MB |
| Item Type: | Article |
|---|---|
| Title: | In vivo single-cell RNA metabolic labeling resolves early transcriptional responders in the regenerating zebrafish heart |
| Creators Name: | Mintcheva, Janita, Tseng, Tzu-Lun, Goumenaki, Pinelopi, Neuschulz, Anika, Senoussi, Anis, Lelek, Sara, Lim, Khai Lone, Ming, Zhitao, Schäfer, Ronny, Hnatiuk, Alisa, Ninov, Nikolay, Beisaw, Arica, McGrail, Maura, Ben Lai, Shih-Lei, Panáková, Daniela, Stainier, Didier Y. R. and Junker, Jan Philipp |
| Abstract: | The adult zebrafish heart can regenerate after injury, but the earliest gene expression changes that trigger this process remain poorly understood. Here we show that in vivo single-cell RNA metabolic labeling, which marks newly made RNA in individual cells, can capture rapid responses in the adult zebraf ish heart after injury. Within the first 6h, we detect activation of innate immune programs, including Toll-like receptor signaling, in a subset of macrophage-like immunecells.Analysisofa largersingle-cell dataset indicates that neutrophils also contribute to this early response. Guided by these data, weshowthat macrophage-specific inhibition of the Toll-like receptor adaptor MyD88 reduces the pro-inflammatory macrophage response at the injury site and improves early hallmarks of regeneration. Our work establishes RNA metabolic labeling as a useful approach for measuring acute responses in vivo at single-cell resolution and identifies early immune-cell activation as a tunable component of heart regeneration. |
| Keywords: | Heart, Innate Immunity, Macrophages, Myeloid Differentiation Factor 88, Myocardium, Neutrophils, RNA, Regeneration, Signal Transduction, Single-Cell Analysis, Toll-Like Receptors, Zebrafish Proteins, Animals, Zebrafish |
| Source: | Nature Communications |
| ISSN: | 2041-1723 |
| Publisher: | Nature Publishing Group |
| Volume: | 17 |
| Number: | 1 |
| Page Range: | 4073 |
| Date: | 5 May 2026 |
| Official Publication: | https://doi.org/10.1038/s41467-026-72781-2 |
| PubMed: | View item in PubMed |
| Related to: |
Repository Staff Only: item control page
